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Development and application of a new scheme of phages for typing and differentiating Salmonella strains from different sources.

D Castro, M A Moriigo, E Martinez-Manzanares, R Cornax, M C Balebona, A Luque and J J Borrego

Department of Microbiology, Faculty of Sciences, University of Malaga, Spain.

ABSTRACT

A set of 25 phages for typing and differentiating Salmonella strains from different origins (food, water, and disease outbreaks) is described. All the strains were typeable by use of the phages, whereas by the serological method more than 5% of the strains could not be classified. By using the phage typing scheme, 75 phage types were established, and all the results were reproducible 1 and 6 months later. Some phages were serotype and serogroup specific, which may be useful in additional tests for the identification of strains of some Salmonella serotypes. In addition, the strains responsible for an outbreak possessed the same phage type, which implies the potential epidemiological use of these phages.