This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gross, U Articles by Heesemann, J Search for Related Content PubMed PubMed Citation Articles by Gross, U Articles by Heesemann, J

 Previous Article  |  Next Article 

J Clin Microbiol. 1992 June; 30(6): 1436-1441

Improved serological diagnosis of Toxoplasma gondii infection by detection of immunoglobulin A (IgA) and IgM antibodies against P30 by using the immunoblot technique.

Institute of Hygiene and Microbiology, University of Würzburg, Germany.

ABSTRACT

Immunoglobulin M (IgM) and IgA antibodies against the major surface protein of Toxoplasma gondii were determined in a total of 195 human sera and five human cerebrospinal fluids by using a P30 membrane extract and the immunoblot technique. By using two different T. gondii strains (RH and BK) simultaneously as antigens, we were able to demonstrate diagnostically important strain-specific human antibody responses in 4.5% of the samples tested. A comparison of the immunoblot technique with an IgM immunocapture enzyme-linked immunosorbent assay demonstrated that the IgM and IgA immunoblot seems to be of advantage in the diagnosis of acute toxoplasmosis in certain groups of patients, especially in the diagnosis of cerebral toxoplasmosis in patients with AIDS. The immunoblot technique described is easy to perform and might be useful as an additional serological assay for routine diagnosis of T. gondii infections.

This article has been cited by other articles:

• Gross, U., Lüder, C. G. K., Hendgen, V., Heeg, C., Sauer, I., Weidner, A., Krczal, D., Enders, G. (2000). Comparative Immunoglobulin G Antibody Profiles between Mother and Child (CGMC Test) for Early Diagnosis of Congenital Toxoplasmosis. J. Clin. Microbiol. 38: 3619-3622 [Abstract] [Full Text]  
• Martin, V., Arcavi, M., Santillan, G., Amendoeira, M. R. R., De Souza Neves, E., Griemberg, G., Guarnera, E., Garberi, J. C., Angel, S. O. (1998). Detection of Human Toxoplasma-Specific Immunoglobulins A, M, and G with a Recombinant Toxoplasma gondii Rop2 Protein. CVI 5: 627-631 [Abstract] [Full Text]