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J Clin Microbiol. 1992 June; 30(6): 1457-1461

Detection of borreliacidal antibodies in hamsters by using flow cytometry.

K F Sachsenmeier, K Schell, L W Morrissey, D R Pennell, R M West, S M Callister and R F Schell

Wisconsin State Laboratory of Hygiene, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison 53706.

ABSTRACT

Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease.

J Clin Microbiol. 1992 June; 30(6): 1457-1461






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1992 by the American Society for Microbiology. All rights reserved.