This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Goh, S H Articles by Chow, A W Search for Related Content PubMed PubMed Citation Articles by Goh, S H Articles by Chow, A W

Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms.

Department of Medicine, University of British Columbia, Vancouver, Canada.

ABSTRACT

Staphylocoagulase, a major phenotypic determinant of Staphylococcus aureus, exists in multiple allelic forms, in part because of the existence of gene variants within the 3'-end coding region. This region contains a series of repeating 81-bp DNA sequences which differ both in the number of tandem repeats and the location of AluI restriction sites among different isolates. Utilizing this finding, we developed a novel typing method for S. aureus based on polymerase chain reaction amplification of the variable region of the coagulase gene followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP). Among 30 S. aureus isolates studied initially, a total of 10 distinct RFLP patterns were observed. There was excellent correlation of the RFLP patterns with typing of these isolates by multilocus enzyme electrophoresis at 20 chromosomal loci. This coagulase RFLP method was used to analyze an additional 39 S. aureus isolates and successfully traced the source of an outbreak of methicillin-resistant S. aureus infections at a local hospital.

This article has been cited by other articles:

• Hussain, M., von Eiff, C., Sinha, B., Joost, I., Herrmann, M., Peters, G., Becker, K. (2008). eap Gene as Novel Target for Specific Identification of Staphylococcus aureus. J. Clin. Microbiol. 46: 470-476 [Abstract] [Full Text]  
• Moon, J.-S., Lee, A.-R., Kang, H.-M., Lee, E.-S., Kim, M.-N., Paik, Y. H., Park, Y. H., Joo, Y.-S., Koo, H. C. (2007). Phenotypic and Genetic Antibiogram of Methicillin-Resistant Staphylococci Isolated from Bovine Mastitis in Korea. J DAIRY SCI 90: 1176-1185 [Abstract] [Full Text]  
• Ishino, K., Tsuchizaki, N., Ishikawa, J., Hotta, K. (2007). Usefulness of PCR-Restriction Fragment Length Polymorphism Typing of the Coagulase Gene To Discriminate Arbekacin-Resistant Methicillin-Resistant Staphylococcus aureus Strains. J. Clin. Microbiol. 45: 607-609 [Abstract] [Full Text]  
• Weist, K., Cimbal, A.-K., Lecke, C., Kampf, G., Ruden, H., Vonberg, R.-P. (2006). Evaluation of six agglutination tests for Staphylococcus aureus identification depending upon local prevalence of meticillin-resistant S. aureus (MRSA).. J Med Microbiol 55: 283-290 [Abstract] [Full Text]  
• Guler, L., Ok, U., Gunduz, K., Gulcu, Y., Hadimli, H. H. (2005). Antimicrobial Susceptibility and Coagulase Gene Typing of Staphylococcus aureus Isolated from Bovine Clinical Mastitis Cases in Turkey. J DAIRY SCI 88: 3149-3154 [Abstract] [Full Text]  
• Watanabe, S., Ito, T., Takeuchi, F., Endo, M., Okuno, E., Hiramatsu, K. (2005). Structural Comparison of Ten Serotypes of Staphylocoagulases in Staphylococcus aureus. J. Bacteriol. 187: 3698-3707 [Abstract] [Full Text]  
• Wertheim, H. F. L., Verveer, J., Boelens, H. A. M., van Belkum, A., Verbrugh, H. A., Vos, M. C. (2005). Effect of Mupirocin Treatment on Nasal, Pharyngeal, and Perineal Carriage of Staphylococcus aureus in Healthy Adults. Antimicrob. Agents Chemother. 49: 1465-1467 [Abstract] [Full Text]  
• Coombs, G. W., Nimmo, G. R., Bell, J. M., Huygens, F., O'Brien, F. G., Malkowski, M. J., Pearson, J. C., Stephens, A. J., Giffard, P. M., the Australian Group for Antimicrobial Resistance, (2004). Genetic Diversity among Community Methicillin-Resistant Staphylococcus aureus Strains Causing Outpatient Infections in Australia. J. Clin. Microbiol. 42: 4735-4743 [Abstract] [Full Text]  
• Stranden, A., Frei, R., Widmer, A. F. (2003). Molecular Typing of Methicillin-Resistant Staphylococcus aureus: Can PCR Replace Pulsed-Field Gel Electrophoresis?. J. Clin. Microbiol. 41: 3181-3186 [Abstract] [Full Text]  
• van Leeuwen, W. B., Jay, C., Snijders, S., Durin, N., Lacroix, B., Verbrugh, H. A., Enright, M. C., Troesch, A., van Belkum, A. (2003). Multilocus Sequence Typing of Staphylococcus aureus with DNA Array Technology. J. Clin. Microbiol. 41: 3323-3326 [Abstract] [Full Text]  
• Sharer, M. V., Su, C., Hegde, N. V., Jayarao, B. M., Sordillo, L. M. (2003). Differential Expression of the Lactose Transporter Gene Affects Growth of Staphylococcus aureus in Milk. J DAIRY SCI 86: 2373-2381 [Abstract] [Full Text]  
• Sabat, A., Krzyszton-Russjan, J., Strzalka, W., Filipek, R., Kosowska, K., Hryniewicz, W., Travis, J., Potempa, J. (2003). New Method for Typing Staphylococcus aureus Strains: Multiple-Locus Variable-Number Tandem Repeat Analysis of Polymorphism and Genetic Relationships of Clinical Isolates. J. Clin. Microbiol. 41: 1801-1804 [Abstract] [Full Text]  
• Adhikari, R. P., Cook, G. M., Lamont, I., Lang, S., Heffernan, H., Smith, J. M. B. (2002). Phenotypic and molecular characterization of community occurring, Western Samoan phage pattern methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 50: 825-831 [Abstract] [Full Text]  
• Montesinos, I., Salido, E., Delgado, T., Cuervo, M., Sierra, A. (2002). Epidemiologic Genotyping of Methicillin-Resistant Staphylococcus aureus by Pulsed-Field Gel Electrophoresis at a University Hospital and Comparison with Antibiotyping and Protein A and Coagulase Gene Polymorphisms. J. Clin. Microbiol. 40: 2119-2125 [Abstract] [Full Text]  
• Donnio, P.-Y., Louvet, L., Preney, L., Nicolas, D., Avril, J.-L., Desbordes, L. (2002). Nine-Year Surveillance of Methicillin-Resistant Staphylococcus aureus in a Hospital Suggests Instability of mecA DNA Region in an Epidemic Strain. J. Clin. Microbiol. 40: 1048-1052 [Abstract] [Full Text]  
• Becker, K., Keller, B., von Eiff, C., Bruck, M., Lubritz, G., Etienne, J., Peters, G. (2001). Enterotoxigenic Potential of Staphylococcus intermedius. Appl. Environ. Microbiol. 67: 5551-5557 [Abstract] [Full Text]  
• Akineden, O., Annemuller, C., Hassan, A. A., Lammler, C., Wolter, W., Zschock, M. (2001). Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis. CVI 8: 959-964 [Abstract] [Full Text]  
• Mason, W. J., Blevins, J. S., Beenken, K., Wibowo, N., Ojha, N., Smeltzer, M. S. (2001). Multiplex PCR Protocol for the Diagnosis of Staphylococcal Infection. J. Clin. Microbiol. 39: 3332-3338 [Abstract] [Full Text]  
• Nimmo, G. R., Schooneveldt, J., O'Kane, G., McCall, B., Vickery, A. (2000). Community Acquisition of Gentamicin-Sensitive Methicillin-Resistant Staphylococcus aureus in Southeast Queensland, Australia. J. Clin. Microbiol. 38: 3926-3931 [Abstract] [Full Text]  
• Shopsin, B., Gomez, M., Waddington, M., Riehman, M., Kreiswirth, B. N. (2000). Use of Coagulase Gene (coa) Repeat Region Nucleotide Sequences for Typing of Methicillin-Resistant Staphylococcus aureus Strains. J. Clin. Microbiol. 38: 3453-3456 [Abstract] [Full Text]  
• Chiou, C.-S., Wei, H.-L., Yang, L.-C. (2000). Comparison of Pulsed-Field Gel Electrophoresis and Coagulase Gene Restriction Profile Analysis Techniques in the Molecular Typing of Staphylococcus aureus. J. Clin. Microbiol. 38: 2186-2190 [Abstract] [Full Text]  
• Tang, Y.-W., Waddington, M. G., Smith, D. H., Manahan, J. M., Kohner, P. C., Highsmith, L. M., Li, H., Cockerill, F. R. III, Thompson, R. L., Montgomery, S. O., Persing, D. H. (2000). Comparison of Protein A Gene Sequencing with Pulsed-Field Gel Electrophoresis and Epidemiologic Data for Molecular Typing of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 38: 1347-1351 [Abstract] [Full Text]  
• VandenBergh, M. F. Q., Yzerman, E. P. F., van Belkum, A., Boelens, H. A. M., Sijmons, M., Verbrugh, H. A. (1999). Follow-Up of Staphylococcus aureus Nasal Carriage after 8 Years: Redefining the Persistent Carrier State. J. Clin. Microbiol. 37: 3133-3140 [Abstract] [Full Text]  
• O'Brien, F. G., Pearman, J. W., Gracey, M., Riley, T. V., Grubb, W. B. (1999). Community Strain of Methicillin-Resistant Staphylococcus aureus Involved in a Hospital Outbreak. J. Clin. Microbiol. 37: 2858-2862 [Abstract] [Full Text]  
• Hsueh, P.-R., Teng, L.-J., Yang, P.-C., Pan, H.-J., Chen, Y.-C., Wang, L.-H., Ho, S.-W., Luh, K.-T. (1999). Dissemination of Two Methicillin-Resistant Staphylococcus aureus Clones Exhibiting Negative Staphylase Reactions in Intensive Care Units. J. Clin. Microbiol. 37: 504-509 [Abstract] [Full Text]  
• van Belkum, A., Scherer, S., van Alphen, L., Verbrugh, H. (1998). Short-Sequence DNA Repeats in Prokaryotic Genomes. Microbiol. Mol. Biol. Rev. 62: 275-293 [Abstract] [Full Text]  
• Hookey, J. V., Richardson, J. F., Cookson, B. D. (1998). Molecular Typing of Staphylococcus aureus Based on PCR Restriction Fragment Length Polymorphism and DNA Sequence Analysis of the Coagulase Gene. J. Clin. Microbiol. 36: 1083-1089 [Abstract] [Full Text]