This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gunzer, F Articles by Karch, H Search for Related Content PubMed PubMed Citation Articles by Gunzer, F Articles by Karch, H

 Previous Article  |  Next Article 

J Clin Microbiol. 1992 July; 30(7): 1807-1810

Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome.

Institut für Hygiene und Mikrobiologie, Universität Würzburg, Germany.

ABSTRACT

Shiga-like toxin-producing Escherichia coli strains of serogroup O157 were identified in 26 of 104 patients with hemolytic-uremic syndrome and in 18 of 668 patients with diarrhea. All strains were identified by colony hybridization with DNA probes complementary to Shiga-like toxin I and Shiga-like toxin II gene sequences and characterized by biochemical tests and serotyping. Seventeen of these 44 patients had E. coli O157 strains which were unusual because they fermented sorbitol within 24 h of incubation and were positive for beta-glucuronidase activity. Culture filtrates of these sorbitol-fermenting strains were highly toxic to Vero cells in culture. Serological tests and DNA analysis performed by restriction endonuclease digestion of B-subunit toxin genes revealed that all 17 isolates produced Shiga-like toxin II. Although by using molecular probes we established a high frequency of sorbitol-fermenting E. coli O157 strains in the patients we examined, further studies on the prevalence of such isolates in other areas of endemic disease are clearly warranted.

This article has been cited by other articles:

• Hajra, T. K., Bag, P. K., Das, S. C., Mukherjee, S., Khan, A., Ramamurthy, T. (2007). Development of a Simple Latex Agglutination Assay for Detection of Shiga Toxin-Producing Escherichia coli (STEC) by Using Polyclonal Antibody against STEC. CVI 14: 600-604 [Abstract] [Full Text]  
• Zhang, W., Qi, W., Albert, T. J., Motiwala, A. S., Alland, D., Hyytia-Trees, E. K., Ribot, E. M., Fields, P. I., Whittam, T. S., Swaminathan, B. (2006). Probing genomic diversity and evolution of Escherichia coli O157 by single nucleotide polymorphisms. Genome Res 16: 757-767 [Abstract] [Full Text]  
• Dobbin, H. S., Hovde, C. J., Williams, C. J., Minnich, S. A. (2006). The Escherichia coli O157 Flagellar Regulatory Gene flhC and Not the Flagellin Gene fliC Impacts Colonization of Cattle.. Infect. Immun. 74: 2894-2905 [Abstract] [Full Text]  
• Nagano, H., Hirochi, T., Fujita, K., Wakamori, Y., Takeshi, K., Yano, S. (2004). Phenotypic and genotypic characterization of {beta}-D-glucuronidase-positive Shiga toxin-producing Escherichia coli O157 : H7 isolates from deer. J Med Microbiol 53: 1037-1043 [Abstract] [Full Text]  
• Monday, S. R., Minnich, S. A., Feng, P. C. H. (2004). A 12-Base-Pair Deletion in the Flagellar Master Control Gene flhC Causes Nonmotility of the Pathogenic German Sorbitol-Fermenting Escherichia coli O157:H- Strains. J. Bacteriol. 186: 2319-2327 [Abstract] [Full Text]  
• Blanco, J. E., Blanco, M., Alonso, M. P., Mora, A., Dahbi, G., Coira, M. A., Blanco, J. (2004). Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin (Verotoxin)-Producing Escherichia coli Isolates from Human Patients: Prevalence in Lugo, Spain, from 1992 through 1999. J. Clin. Microbiol. 42: 311-319 [Abstract] [Full Text]  
• Shaikh, N., Tarr, P. I. (2003). Escherichia coli O157:H7 Shiga Toxin-Encoding Bacteriophages: Integrations, Excisions, Truncations, and Evolutionary Implications. J. Bacteriol. 185: 3596-3605 [Abstract] [Full Text]  
• Blanco, J., Blanco, M., Blanco, J. E., Mora, A., Gonzalez, E. A., Bernardez, M. I., Alonso, M. P., Coira, A., Rodriguez, A., Rey, J., Alonso, J. M., Usera, M. A. (2003). Verotoxin-Producing Escherichia coli in Spain: Prevalence, Serotypes, and Virulence Genes of O157:H7 and Non-O157 VTEC in Ruminants, Raw Beef Products, and Humans. Exp. Biol. Med. 228: 345-351 [Abstract] [Full Text]  
• Ludwig, K., Sarkim, V., Bitzan, M., Karmali, M. A., Bobrowski, C., Ruder, H., Laufs, R., Sobottka, I., Petric, M., Karch, H., Muller-Wiefel, D. E. (2002). Shiga Toxin-Producing Escherichia coli Infection and Antibodies against Stx2 and Stx1 in Household Contacts of Children with Enteropathic Hemolytic-Uremic Syndrome. J. Clin. Microbiol. 40: 1773-1782 [Abstract] [Full Text]  
• NAGANO, H., OKUI, T., FUJIWARA, O., UCHIYAMA, Y., TAMATE, N., KUMADA, H., MORIMOTO, Y., YANO, S. (2002). Clonal structure of Shiga toxin (Stx)-producing and {beta}-D-glucuronidase-positive Escherichia coli O157:H7 strains isolated from outbreaks and sporadic cases in Hokkaido, Japan. J Med Microbiol 51: 405-416 [Abstract] [Full Text]  
• de Vaux, A., Morrison, M., Hutkins, R. W. (2002). Displacement of Escherichia coli O157:H7 from Rumen Medium Containing Prebiotic Sugars. Appl. Environ. Microbiol. 68: 519-524 [Abstract] [Full Text]  
• Kim, J., Nietfeldt, J., Ju, J., Wise, J., Fegan, N., Desmarchelier, P., Benson, A. K. (2001). Ancestral Divergence, Genome Diversification, and Phylogeographic Variation in Subpopulations of Sorbitol-Negative, beta -Glucuronidase-Negative Enterohemorrhagic Escherichia coli O157. J. Bacteriol. 183: 6885-6897 [Abstract] [Full Text]  
• Brunder, W., Khan, A. S., Hacker, J., Karch, H. (2001). Novel Type of Fimbriae Encoded by the Large Plasmid of Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H{-}. Infect. Immun. 69: 4447-4457 [Abstract] [Full Text]  
• Karch, H., Bielaszewska, M. (2001). Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H{-} Strains: Epidemiology, Phenotypic and Molecular Characteristics, and Microbiological Diagnosis. J. Clin. Microbiol. 39: 2043-2049 [Full Text]  
• Ludwig, K., Karmali, M. A., Sarkim, V., Bobrowski, C., Petric, M., Karch, H., Muller-Wiefel, D. E., Arbeitsgemeinschaft fur Padiatrische Nephrologie, (2001). Antibody Response to Shiga Toxins Stx2 and Stx1 in Children with Enteropathic Hemolytic-Uremic Syndrome. J. Clin. Microbiol. 39: 2272-2279 [Abstract] [Full Text]  
• Bielaszewska, M., Schmidt, H., Liesegang, A., Prager, R., Rabsch, W., Tschäpe, H., Cízek, A., Janda, J., Bláhová, K., Karch, H. (2000). Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H- Strains and a Source of Human Diseases. J. Clin. Microbiol. 38: 3470-3473 [Abstract] [Full Text]  
• Zhang, W.-L., Bielaszewska, M., Bockemühl, J., Schmidt, H., Scheutz, F., Karch, H. (2000). Molecular Analysis of H Antigens Reveals that Human Diarrheagenic Escherichia coli O26 Strains That Carry the eae Gene Belong to the H11 Clonal Complex. J. Clin. Microbiol. 38: 2989-2993 [Abstract] [Full Text]  
• Zhang, W.-L., Bielaszewska, M., Liesegang, A., Tschäpe, H., Schmidt, H., Bitzan, M., Karch, H. (2000). Molecular Characteristics and Epidemiological Significance of Shiga Toxin-Producing Escherichia coli O26 Strains. J. Clin. Microbiol. 38: 2134-2140 [Abstract] [Full Text]  
• Köhler, B., Karch, H., Schmidt, H. (2000). Antibacterials that are used as growth promoters in animal husbandry can affect the release of Shiga-toxin-2-converting bacteriophages and Shiga toxin 2 from Escherichia coli strains. Microbiology 146: 1085-1090 [Abstract] [Full Text]  
• Novicki, T. J., Daly, J. A., Mottice, S. L., Carroll, K. C. (2000). Comparison of Sorbitol MacConkey Agar and a Two-Step Method Which Utilizes Enzyme-Linked Immunosorbent Assay Toxin Testing and a Chromogenic Agar To Detect and Isolate Enterohemorrhagic Escherichia coli. J. Clin. Microbiol. 38: 547-551 [Abstract] [Full Text]  
• Oswald, E., Schmidt, H., Morabito, S., Karch, H., Marches, O., Caprioli, A. (2000). Typing of Intimin Genes in Human and Animal Enterohemorrhagic and Enteropathogenic Escherichia coli: Characterization of a New Intimin Variant. Infect. Immun. 68: 64-71 [Abstract] [Full Text]  
• Karch, H., Schubert, S., Zhang, D., Zhang, W., Schmidt, H., Olschlager, T., Hacker, J. (1999). A Genomic Island, Termed High-Pathogenicity Island, Is Present in Certain Non-O157 Shiga Toxin-Producing Escherichia coli Clonal Lineages. Infect. Immun. 67: 5994-6001 [Abstract] [Full Text]  
• Schmidt, H., Scheef, J., Huppertz, H. I., Frosch, M., Karch, H. (1999). Escherichia coli O157:H7 and O157:H- Strains That Do Not Produce Shiga Toxin: Phenotypic and Genetic Characterization of Isolates Associated with Diarrhea and Hemolytic-Uremic Syndrome. J. Clin. Microbiol. 37: 3491-3496 [Abstract] [Full Text]  
• Plunkett, G. III, Rose, D. J., Durfee, T. J., Blattner, F. R. (1999). Sequence of Shiga Toxin 2 Phage 933W from Escherichia coli O157:H7: Shiga Toxin as a Phage Late-Gene Product. J. Bacteriol. 181: 1767-1778 [Abstract] [Full Text]  
• Keskimäki, M., Saari, M., Heiskanen, T., Siitonen, A. (1998). Shiga Toxin-Producing Escherichia coli in Finland from 1990 through 1997: Prevalence and Characteristics of Isolates. J. Clin. Microbiol. 36: 3641-3646 [Abstract] [Full Text]  
• Oberst, R. D., Hays, M. P., Bohra, L. K., Phebus, R. K., Yamashiro, C. T., Paszko-Kolva, C., Flood, S. J. A., Sargeant, J. M., Gillespie, J. R. (1998). PCR-Based DNA Amplification and Presumptive Detection of Escherichia coli O157:H7 with an Internal Fluorogenic Probe and the 5' Nuclease (TaqMan) Assay. Appl. Environ. Microbiol. 64: 3389-3396 [Abstract] [Full Text]  
• Paton, J. C., Paton, A. W. (1998). Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections. Clin. Microbiol. Rev. 11: 450-479 [Abstract] [Full Text]  
• Bielaszewska, M., Schmidt, H., Karmali, M. A., Khakhria, R., Janda, J., Karch, H. (1998). Isolation and Characterization of Sorbitol-Fermenting Shiga Toxin (Verocytotoxin)-Producing Escherichia coli O157:Hsstrains in the Czech Republic. J. Clin. Microbiol. 36: 2135-2137 [Abstract] [Full Text]  
• Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]