This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Soini, H Articles by Viljanen, M K Search for Related Content PubMed PubMed Citation Articles by Soini, H Articles by Viljanen, M K

Detection and identification of mycobacteria by amplification of a segment of the gene coding for the 32-kilodalton protein.

Department of Turku, National Public Health Institute, Finland.

ABSTRACT

A polymerase chain reaction (PCR) assay for the rapid detection of mycobacterial DNA is described. Oligonucleotide primers, derived from the sequence of a gene coding for the 32-kDa antigen of Mycobacterium tuberculosis, amplified DNA from all 28 species of mycobacteria tested. All nonmycobacterial species tested were negative. An oligonucleotide probe hybridized to the PCR products of the strains belonging to the M. tuberculosis complex. This method could detect as little as 50 fg, as tested with purified M. tuberculosis DNA. By this amplification method, 127 sputum specimens were tested, with 7.9% of the specimens proving to be inhibitory in PCR. The sensitivity of detection by PCR compared with that by culture was 55.9%; when the inhibitory specimens were excluded, the sensitivity was 70.4%. The specificity of PCR combined with hybridization was 100%.

This article has been cited by other articles:

• Kainulainen, L., Suonpaa, J., Nikoskelainen, J., Svedstrom, E., Vuorinen, T., Meurman, O., Ruuskanen, O. (2007). Bacteria and Viruses in Maxillary Sinuses of Patients With Primary Hypogammaglobulinemia. Arch Otolaryngol Head Neck Surg 133: 597-602 [Abstract] [Full Text]  
• Lima, D. M., Colares, J. K. B., da Fonseca, B. A. L. (2003). Combined Use of the Polymerase Chain Reaction and Detection of Adenosine Deaminase Activity on Pleural Fluid Improves the Rate of Diagnosis of Pleural Tuberculosis. Chest 124: 909-914 [Abstract] [Full Text]  
• Kaul, K. L. (2001). Molecular Detection of Mycobacterium tuberculosis: Impact on Patient Care. Clin. Chem. 47: 1553-1558 [Abstract] [Full Text]  
• Yaramis, A., Tekes, S., Bilici, M. (2000). Diagnostic Performance of Amplified Mycobacterium Tuberculosis Direct Test in the Cerebrospinal Fluid of Children With Tuberculosis Meningitis. Pediatrics 106: 162a-162 [Full Text]  
• KAINULAINEN, L., NIKOSKELAINEN, J., VUORINEN, T., TEVOLA, K., LIIPPO, K., RUUSKANEN, O. (1999). Viruses and Bacteria in Bronchial Samples from Patients with Primary Hypogammaglobulinemia. Am. J. Respir. Crit. Care Med. 159: 1199-1204 [Abstract] [Full Text]  
• Baumeister, A. K., Runge, M., Ganter, M., Feenstra, A. A., Delbeck, F., Kirchhoff, H. (1998). Detection of Mycoplasma hyopneumoniae in Bronchoalveolar Lavage Fluids of Pigs by PCR. J. Clin. Microbiol. 36: 1984-1988 [Abstract] [Full Text]  
• Gamboa, F., Dominguez, J., Padilla, E., Manterola, J. M., Gazapo, E., Lonca, J., Matas, L., Hernandez, A., Cardona, P. J., Ausina, V. (1998). Rapid Diagnosis of Extrapulmonary Tuberculosis by Ligase Chain Reaction Amplification. J. Clin. Microbiol. 36: 1324-1329 [Abstract] [Full Text]  
• Leckie, G. W., Erickson, D. D., He, Q., Facey, I. E., Lin, B.-C., Cao, J., Halaka, F. G. (1998). Method for Reduction of Inhibition in a Mycobacterium tuberculosis-Specific Ligase Chain Reaction DNA Amplification Assay. J. Clin. Microbiol. 36: 764-767 [Abstract] [Full Text]