This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chaicumpa, W Articles by Echeverria, P Search for Related Content PubMed PubMed Citation Articles by Chaicumpa, W Articles by Echeverria, P

 Previous Article  |  Next Article 

J Clin Microbiol. 1992 September; 30(9): 2513-2515

Diagnosis of typhoid fever by detection of Salmonella typhi antigen in urine.

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

ABSTRACT

A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Salmonella typhi was isolated from hemocultures, 73% in patients in whom S. typhi was isolated from stool specimens, and 40% in patients in whom the organism was isolated from bone marrow cultures. Among patients in whom S. typhi was isolated from blood cultures, the ELISA had a sensitivity of 65% when a single urine specimen was examined and 95% when serially collected urine specimens were examined. A dot blot immunoassay performed on a nitrocellulose filter in parallel had a sensitivity of 85%, versus 83% for the plate ELISA in which S. typhi was isolated from blood, bone marrow, and/or stool specimens. Since S. typhi antigen is intermittently excreted in the urine of patients with typhoid fever, serially collected urine from patients with typhoid should be tested for antigen 9.

This article has been cited by other articles:

• Tam, F. C. H., Ling, T. K. W., Wong, K. T., Leung, D. T. M., Chan, R. C. Y., Lim, P. L. (2008). The TUBEX test detects not only typhoid-specific antibodies but also soluble antigens and whole bacteria. J Med Microbiol 57: 316-323 [Abstract] [Full Text]  
• HATTA, M., SMITS, H. L. (2007). DETECTION OF SALMONELLA TYPHI BY NESTED POLYMERASE CHAIN REACTION IN BLOOD, URINE, AND STOOL SAMPLES. Am J Trop Med Hyg 76: 139-143 [Abstract] [Full Text]  
• FADEEL, M. A., CRUMP, J. A., MAHONEY, F. J., NAKHLA, I. A., MANSOUR, A. M., REYAD, B., EL MELEGI, D., SULTAN, Y., MINTZ, E. D., BIBB, W. F. (2004). RAPID DIAGNOSIS OF TYPHOID FEVER BY ENZYME-LINKED IMMUNOSORBENT ASSAY DETECTION OF SALMONELLA SEROTYPE TYPHI ANTIGENS IN URINE. Am J Trop Med Hyg 70: 323-328 [Abstract] [Full Text]  
• Saengjaruk, P., Chaicumpa, W., Watt, G., Bunyaraksyotin, G., Wuthiekanun, V., Tapchaisri, P., Sittinont, C., Panaphut, T., Tomanakan, K., Sakolvaree, Y., Chongsa-Nguan, M., Mahakunkijcharoen, Y., Kalambaheti, T., Naigowit, P., Wambangco, M. A. L., Kurazono, H., Hayashi, H. (2002). Diagnosis of Human Leptospirosis by Monoclonal Antibody-Based Antigen Detection in Urine. J. Clin. Microbiol. 40: 480-489 [Abstract] [Full Text]  
• Lim, P.-L., Tam, F. C. H., Cheong, Y.-M., Jegathesan, M. (1998). One-Step 2-Minute Test To Detect Typhoid-Specific Antibodies Based on Particle Separation in Tubes. J. Clin. Microbiol. 36: 2271-2278 [Abstract] [Full Text]