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Growth and phenotypic characterization of Legionella species on semisolid media made with washed agar.

Department of Microbiology and Immunology, University of Michigan, Ann Arbor 48109-0620.

ABSTRACT

Legionella pneumophila (and 28 Legionella species) grew efficiently on charcoal-free, buffered yeast extract medium made with washed agar and without apparent loss of infectivity for U937 cells. Because charcoal-free, buffered yeast extract is transparent, it is a suitable base for indicator media and pigment detection. In standard media, charcoal apparently prevents agar contaminants from inhibiting Legionella growth.