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J Clin Microbiol. 1993 January; 31(1): 152-154

Detection of Francisella tularensis in blood by polymerase chain reaction.

G W Long, J J Oprandy, R B Narayanan, A H Fortier, K R Porter and C A Nacy

Center for Immunization Research, Johns Hopkins University, Baltimore, Maryland 21205.

ABSTRACT

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

J Clin Microbiol. 1993 January; 31(1): 152-154




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1993 by the American Society for Microbiology. All rights reserved.