This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wallace, R J Articles by Maloney, S Search for Related Content PubMed PubMed Citation Articles by Wallace, R J, Jr Articles by Maloney, S

DNA large restriction fragment patterns of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus.

Department of Microbiology, University of Texas Health Center, Tyler 75710.

ABSTRACT

Large restriction fragment (LRF) pattern analysis of genomic DNA using pulsed-field gel electrophoresis was performed on three reference strains, 32 sporadic isolates, and 92 nosocomial isolates from 12 epidemics of Mycobacterium chelonae and Mycobacterium abscessus. Only 17 of 30 (57%) unrelated strains of M. abscessus, compared with 10 of 11 (91%) of M. chelonae strains, gave satisfactory DNA extractions, with the remainder resulting in highly fragmented DNA. DraI, AsnI, XbaI, and SpeI gave satisfactory LRF patterns. Sporadic isolates of the two species had highly variable LRF patterns, except for one reference strain and one sporadic isolate of M. chelonae that differed by only two to five bands. Evaluation of repeat isolates from five patients monitored for 8 months to 13 years (mean, 5.8 years) revealed LRF patterns to be stable, with changes of not more than two bands. LRF analysis of the seven nosocomial outbreaks with evaluable DNA revealed identical patterns in most or all of the patient isolates and in three outbreaks revealed identity with environmental isolates. These outbreaks included endoscope contamination, postinjection abscesses, and surgical wound infections. LRF analysis of genomic DNA is a useful technique for epidemiologic studies of M. abscessus and M. chelonae, although improved technology is needed for the approximately 50% of strains of M. abscessus with unsatisfactory DNA extractions.

This article has been cited by other articles:

• Rottman, M., Catherinot, E., Hochedez, P., Emile, J.-F., Casanova, J.-L., Gaillard, J.-L., Soudais, C. (2007). Importance of T Cells, Gamma Interferon, and Tumor Necrosis Factor in Immune Control of the Rapid Grower Mycobacterium abscessus in C57BL/6 Mice. Infect. Immun. 75: 5898-5907 [Abstract] [Full Text]  
• Jonsson, B. E., Gilljam, M., Lindblad, A., Ridell, M., Wold, A. E., Welinder-Olsson, C. (2007). Molecular Epidemiology of Mycobacterium abscessus, with Focus on Cystic Fibrosis. J. Clin. Microbiol. 45: 1497-1504 [Abstract] [Full Text]  
• Zhang, Y., Yakrus, M. A., Graviss, E. A., Williams-Bouyer, N., Turenne, C., Kabani, A., Wallace, R. J. Jr. (2004). Pulsed-Field Gel Electrophoresis Study of Mycobacterium abscessus Isolates Previously Affected by DNA Degradation. J. Clin. Microbiol. 42: 5582-5587 [Abstract] [Full Text]  
• Zhang, Y., Mann, L. B., Wilson, R. W., Brown-Elliott, B. A., Vincent, V., Iinuma, Y., Wallace, R. J. Jr. (2004). Molecular Analysis of Mycobacterium kansasii Isolates from the United States. J. Clin. Microbiol. 42: 119-125 [Abstract] [Full Text]  
• Olivier, K. N., Weber, D. J., Wallace, R. J. Jr., Faiz, A. R., Lee, J.-H., Zhang, Y., Brown-Elliot, B. A., Handler, A., Wilson, R. W., Schechter, M. S., Edwards, L. J., Chakraborti, S., Knowles, M. R. (2003). Nontuberculous Mycobacteria: I: Multicenter Prevalence Study in Cystic Fibrosis. Am. J. Respir. Crit. Care Med. 167: 828-834 [Abstract] [Full Text]  
• Wallace, R. J. Jr., Zhang, Y., Wilson, R. W., Mann, L., Rossmoore, H. (2002). Presence of a Single Genotype of the Newly Described Species Mycobacterium immunogenum in Industrial Metalworking Fluids Associated with Hypersensitivity Pneumonitis. Appl. Environ. Microbiol. 68: 5580-5584 [Abstract] [Full Text]  
• Brown-Elliott, B. A., Wallace, R. J. Jr. (2002). Clinical and Taxonomic Status of Pathogenic Nonpigmented or Late-Pigmenting Rapidly Growing Mycobacteria. Clin. Microbiol. Rev. 15: 716-746 [Abstract] [Full Text]  
• Howard, S. T., Byrd, T. F., Lyons, C. R. (2002). A polymorphic region in Mycobacterium abscessus contains a novel insertion sequence element. Microbiology 148: 2987-2996 [Abstract] [Full Text]  
• Ringuet, H., Akoua-Koffi, C., Honore, S., Varnerot, A., Vincent, V., Berche, P., Gaillard, J. L., Pierre-Audigier, C. (1999). hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria. J. Clin. Microbiol. 37: 852-857 [Abstract] [Full Text]  
• (1997). Diagnosis and Treatment of Disease Caused by Nontuberculous Mycobacteria. Am. J. Respir. Crit. Care Med. 156: 1-25 [Full Text]