This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tamburrini, E Articles by Vicari, G Search for Related Content PubMed PubMed Citation Articles by Tamburrini, E Articles by Vicari, G

 Previous Article  |  Next Article 

J Clin Microbiol. 1993 October; 31(10): 2788-2789

Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii infection.

Department of Infectious Diseases, Università Cattolica, Rome, Italy.

ABSTRACT

Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.

This article has been cited by other articles:

• Wakefield, A. E (2002). Pneumocystis carinii: Role in childhood respiratory infections. Br Med Bull 61: 175-188 [Abstract] [Full Text]  
• Sing, A., Trebesius, K., Roggenkamp, A., Rüssmann, H., Tybus, K., Pfaff, F., Bogner, J. R., Emminger, C., Heesemann, J. (2000). Evaluation of Diagnostic Value and Epidemiological Implications of PCR for Pneumocystis carinii in Different Immunosuppressed and Immunocompetent Patient Groups. J. Clin. Microbiol. 38: 1461-1467 [Abstract] [Full Text]