This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Corney, B G Articles by Graham, G C Search for Related Content PubMed PubMed Citation Articles by Corney, B G Articles by Graham, G C

Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting.

Animal Research Institute, Queensland Department of Primary Industries, Moorooka, Australia.

ABSTRACT

We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Australian cattle. Each serovar and genotype had a unique RAPD profile. Of 26 field isolates of Leptospira, 23 were identified as hardjo genotype bovis subtype A, 2 were identified as zanoni, and 1 was identified as pomona by RAPD fingerprinting, and their types were confirmed by cross-absorption agglutination and restriction enzyme analysis.

This article has been cited by other articles:

• O'Carroll, M R, Kidd, T J, Coulter, C, Smith, H V, Rose, B R, Harbour, C, Bell, S C (2003). Burkholderia pseudomallei: another emerging pathogen in cystic fibrosis. Thorax 58: 1087-1091 [Abstract] [Full Text]  
• Oliveira, M. A. A., Caballero, O. L., Vago, A. R., Harskeerl, R. A., Romanha, A. J., Pena, S. D. J., Simpson, A. J. G., Koury, M. C. (2003). Low-stringency single specific primer PCR for identification of Leptospira. J Med Microbiol 52: 127-135 [Abstract] [Full Text]  
• Levett, P. N. (2001). Leptospirosis. Clin. Microbiol. Rev. 14: 296-326 [Abstract] [Full Text]