This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Steed, L L Articles by Daly, J A Search for Related Content PubMed PubMed Citation Articles by Steed, L L Articles by Daly, J A

Rapid detection of Streptococcus pyogenes in pediatric patient specimens by DNA probe.

Primary Children's Medical Center, University of Utah School of Medicine, Salt Lake City 84132.

ABSTRACT

A chemiluminescent DNA probe test (Group A Streptococcus Direct Test; Gen-Probe, Inc., San Diego, Calif.) for rapid, direct detection of cRNA of Streptococcus pyogenes in throat swabs was compared with conventional culture and identification techniques. Throat swabs from 277 patients suspected of having streptococcal pharyngitis were examined. By DNA probe alone, 10 specimens were positive, 51 were positive by both assays, and 8 were positive by culture alone. Thus, DNA probe sensitivity, specificity, and positive and negative predictive values were 86, 95, 84, and 96%, respectively. Including an indeterminate category, sensitivity, specificity, and positive and negative predictive values were 89, 96, 86, and 97%, respectively. After discrepancy testing, these values for the raw data improved to 90, 98, 93, and 97%, respectively. None of the 24 specimens that grew non-S. pyogenes beta-hemolytic streptococci in culture were positive by the DNA probe. Because mucoid S. pyogenes strains are more virulent than nonmucoid strains, 24 isolates were retrospectively tested with the DNA probe to ensure that both types would be detected equally well. Isolates were examined in pure cultures as well as mixed with representative normal oral flora. There was no statistical difference in detection of any of the four groups. Group A Streptococcus Direct Test is a rapid, sensitive, and specific test for S. pyogenes.

This article has been cited by other articles:

• Gerber, M. A., Shulman, S. T. (2004). Rapid Diagnosis of Pharyngitis Caused by Group A Streptococci. Clin. Microbiol. Rev. 17: 571-580 [Abstract] [Full Text]  
• Richardson, H., Smaill, F. (1998). Recent advances: Medical microbiology. BMJ 317: 1060-1062 [Full Text]