This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Helbig, M Articles by Frosch, M Search for Related Content PubMed PubMed Citation Articles by Helbig, M Articles by Frosch, M

Serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens.

Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.

ABSTRACT

Two recombinant antigens of the larval stages of Echinococcus granulosus and Echinococcus multilocularis, termed EG55 and EM10, respectively, were applied for serodiagnosis and serological differentiation between parasitic infections caused by the metacestode tissue of both tapeworms. Antigen EM10 is synthesized by E. multilocularis larvae. Antigen EG55 represents the recombinant form of the low-molecular-weight subunit of antigen B, which is an Echinococcus genus-specific antigen. Both recombinant antigens were expressed as glutathione S-transferase fusion proteins. A sandwich enzyme-linked immunosorbent assay with monoclonal antibodies against EM10 and EG55 as capture reagents for the recombinant antigens was established and was evaluated with 74 serum samples from patients with histologically confirmed alveolar echinococcosis and 63 serum samples from patients with histologically confirmed cystic echinococcosis. A sensitivity of 93.2% and a specificity of 96.8% were achieved for the serodiagnosis of alveolar echinococcosis. Cystic echinococcosis could be detected with a sensitivity of 89.1% and a specificity of 98.6%.

This article has been cited by other articles:

• Tappe, D., Brehm, K., Frosch, M., Blankenburg, A., Schrod, A., Kaup, F.-J., Matz-Rensing, K. (2007). Echinococcus multilocularis Infection of Several Old World Monkey Species in a Breeding Enclosure. Am J Trop Med Hyg 77: 504-506 [Abstract] [Full Text]  
• Korkmaz, M., Inceboz, T., Celebi, F., Babaoglu, A., Uner, A. (2004). Use of Two Sensitive and Specific Immunoblot Markers, Em70 and Em90, for Diagnosis of Alveolar Echinococcosis. J. Clin. Microbiol. 42: 3350-3352 [Abstract] [Full Text]  
• Zhang, W., Li, J., McManus, D. P. (2003). Concepts in Immunology and Diagnosis of Hydatid Disease. Clin. Microbiol. Rev. 16: 18-36 [Abstract] [Full Text]  
• Sako, Y., Nakao, M., Nakaya, K., Yamasaki, H., Gottstein, B., Lightowers, M. W., Schantz, P. M., Ito, A. (2002). Alveolar Echinococcosis: Characterization of Diagnostic Antigen Em18 and Serological Evaluation of Recombinant Em18. J. Clin. Microbiol. 40: 2760-2765 [Abstract] [Full Text]