This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gretch, D R Articles by Corey, L Search for Related Content PubMed PubMed Citation Articles by Gretch, D R Articles by Corey, L

Detection of hepatitis C virus RNA: comparison of one-stage polymerase chain reaction (PCR) with nested-set PCR.

Department of Laboratory Medicine, University of Washington Medical Center, Seattle 98195.

ABSTRACT

We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens were used. One-stage PCR may be less prone to contamination than nested-set PCR.

This article has been cited by other articles:

• Nolte, F. S., Fried, M. W., Shiffman, M. L., Ferreira-Gonzalez, A., Garrett, C. T., Schiff, E. R., Polyak, S. J., Gretch, D. R. (2001). Prospective Multicenter Clinical Evaluation of AMPLICOR and COBAS AMPLICOR Hepatitis C Virus Tests. J. Clin. Microbiol. 39: 4005-4012 [Abstract] [Full Text]  
• Chang, M., Marquardt, A. P., Wood, B. L., Williams, O., Cotler, S. J., Taylor, S. L., Carithers, R. L. Jr., Gretch, D. R. (2000). In Situ Distribution of Hepatitis C Virus Replicative-Intermediate RNA in Hepatic Tissue and Its Correlation with Liver Disease. J. Virol. 74: 944-955 [Abstract] [Full Text]  
• Sullivan, D. G., Wilson, J. J., Carithers, R. L. Jr., Perkins, J. D., Gretch, D. R. (1998). Multigene Tracking of Hepatitis C Virus Quasispecies after Liver Transplantation: Correlation of Genetic Diversification in the Envelope Region with Asymptomatic or Mild Disease Patterns. J. Virol. 72: 10036-10043 [Abstract] [Full Text]  
• Polyak, S. J., McArdle, S., Liu, S.-L., Sullivan, D. G., Chung, M., Hofgartner, W. T., Carithers, R. L. Jr., McMahon, B. J., Mullins, J. I., Corey, L., Gretch, D. R. (1998). Evolution of Hepatitis C Virus Quasispecies in Hypervariable Region 1 and the Putative Interferon Sensitivity-Determining Region during Interferon Therapy and Natural Infection. J. Virol. 72: 4288-4296 [Abstract] [Full Text]