This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lew, A E Articles by Desmarchelier, P M Search for Related Content PubMed PubMed Citation Articles by Lew, A E Articles by Desmarchelier, P M

 Previous Article  |  Next Article 

J Clin Microbiol. 1993 March; 31(3): 533-539

Molecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphisms of rRNA genes.

Department of Microbiology, University of Queensland, St. Lucia, Australia.

ABSTRACT

The aim of this study was to develop a typing scheme for Pseudomonas pseudomallei by comparison of patterns of restriction fragment length polymorphisms in rRNA genes (ribotyping). BamHI restriction digests of 100 isolates from various animal (34), human (58), and environmental (6) sources, including six reference strains, were hybridized to Escherichia coli 16S and 23S rRNAs. A chemiluminescent labelling and detection system was used to visualize bands. On the basis of patterns, the strains were classified into 22 different groups, with the largest containing 29 isolates. While most of the ribotypes were not exclusive to a particular source, some ribotypes were restricted to a particular geographic area or to either a human or a particular animal species. Application of the typing scheme to isolates of four independent outbreaks among animals showed that certain ribotypes predominated. The study demonstrated ribotyping to be a useful tool in epidemiological investigations of melioidosis.

This article has been cited by other articles:

• Gee, J. E., Sacchi, C. T., Glass, M. B., De, B. K., Weyant, R. S., Levett, P. N., Whitney, A. M., Hoffmaster, A. R., Popovic, T. (2003). Use of 16S rRNA Gene Sequencing for Rapid Identification and Differentiation of Burkholderia pseudomallei and B. mallei. J. Clin. Microbiol. 41: 4647-4654 [Abstract] [Full Text]  
• Inglis, T. J. J., O'Reilly, L., Foster, N., Clair, A., Sampson, J. (2002). Comparison of Rapid, Automated Ribotyping and DNA Macrorestriction Analysis of Burkholderia pseudomallei. J. Clin. Microbiol. 40: 3198-3203 [Abstract] [Full Text]  
• BROWN, N. F., BEACHAM, I. R. (2000). Cloning and analysis of genomic differences unique to Burkholderia pseudomallei by comparison with B. thailandensis. J Med Microbiol 49: 993-1001 [Abstract] [Full Text]  
• Morrison, D., Woodford, N., Barrett, S. P., Sisson, P., Cookson, B. D. (1999). DNA Banding Pattern Polymorphism in Vancomycin-Resistant Enterococcus faecium and Criteria for Defining Strains. J. Clin. Microbiol. 37: 1084-1091 [Abstract] [Full Text]