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J Clin Microbiol. 1993 April; 31(4): 972-974

Rapid screening test for enterotoxin-producing Bacillus cereus.

Hamilton Regional Public Health Laboratory, Ontario Ministry of Health, Canada.

ABSTRACT

Culture supernatants of 30 enterotoxin-producing Bacillus cereus isolates produced a characteristic progressive destruction of McCoy cell monolayers. Enterotoxin-negative B. cereus and other group 1 Bacillus spp. caused no monolayer disruption. The McCoy cell tissue culture system appears to provide a rapid screening assay for detection of enterotoxin-producing B. cereus.

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