This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fournier, J M
Right arrow Articles by Slizewicz, B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fournier, J M
Right arrow Articles by Slizewicz, B

 Previous Article  |  Next Article 

J Clin Microbiol. 1993 May; 31(5): 1342-1344

New latex reagent using monoclonal antibodies to capsular polysaccharide for reliable identification of both oxacillin-susceptible and oxacillin-resistant Staphylococcus aureus.

J M Fournier, A Bouvet, D Mathieu, F Nato, A Boutonnier, R Gerbal, P Brunengo, C Saulnier, N Sagot and B Slizewicz

Unité Choléra et Vibrions, Institut Pasteur, Paris, France.

ABSTRACT

A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotype 5 and 8 capsular polysaccharides, was compared with three commercially available rapid agglutination methods for the identification of 220 isolates of S. aureus (61 oxacillin resistant) and 128 isolates of coagulase-negative staphylococci. The sensitivity for identification of S. aureus was high with the Pastorex Staph-Plus test (98.6%) compared with those of the other tests, which ranged from 91.8 to 84.5%. Test sensitivities for the identification of oxacillin-resistant S. aureus were as follows: Pastorex Staph-Plus, 95.1%; Pastorex Staph, 73.8%; Staphyslide, 72.1%; and StaphAurex, 49.2%.

J Clin Microbiol. 1993 May; 31(5): 1342-1344




This article has been cited by other articles:

  • Weist, K., Cimbal, A.-K., Lecke, C., Kampf, G., Ruden, H., Vonberg, R.-P. (2006). Evaluation of six agglutination tests for Staphylococcus aureus identification depending upon local prevalence of meticillin-resistant S. aureus (MRSA).. J Med Microbiol 55: 283-290 [Abstract] [Full Text]  
  • Blake, J. E., Metcalfe, M. A. (2001). A Shared Noncapsular Antigen Is Responsible for False-Positive Reactions by Staphylococcus epidermidis in Commercial Agglutination Tests for Staphylococcus aureus. J. Clin. Microbiol. 39: 544-550 [Abstract] [Full Text]  
  • van Griethuysen, A., Bes, M., Etienne, J., Zbinden, R., Kluytmans, J. (2001). International Multicenter Evaluation of Latex Agglutination Tests for Identification of Staphylococcus aureus. J. Clin. Microbiol. 39: 86-89 [Abstract] [Full Text]  
  • Gaillot, O., Wetsch, M., Fortineau, N., Berche, P. (2000). Evaluation of CHROMagar Staph. aureus, a New Chromogenic Medium, for Isolation and Presumptive Identification of Staphylococcus aureus from Human Clinical Specimens. J. Clin. Microbiol. 38: 1587-1591 [Abstract] [Full Text]  
  • Hsueh, P.-R., Teng, L.-J., Yang, P.-C., Pan, H.-J., Chen, Y.-C., Wang, L.-H., Ho, S.-W., Luh, K.-T. (1999). Dissemination of Two Methicillin-Resistant Staphylococcus aureus Clones Exhibiting Negative Staphylase Reactions in Intensive Care Units. J. Clin. Microbiol. 37: 504-509 [Abstract] [Full Text]  
  • Wichelhaus, T. A., Kern, S., Schäfer, V., Brade, V. (1999). Rapid Detection of Epidemic Strains of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 37: 690-693 [Abstract] [Full Text]  
  • Bascomb, S., Manafi, M. (1998). Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci. Clin. Microbiol. Rev. 11: 318-340 [Abstract] [Full Text]  
  • Smole, S. C., Aronson, E., Durbin, A., Brecher, S. M., Arbeit, R. D. (1998). Sensitivity and Specificity of an Improved Rapid Latex Agglutination Test for Identification of Methicillin-Sensitive and -Resistant Staphylococcus aureus Isolates. J. Clin. Microbiol. 36: 1109-1112 [Abstract] [Full Text]  
  • Martineau, F., Picard, F. J., Roy, P. H., Ouellette, M., Bergeron, M. G. (1998). Species-Specific and Ubiquitous-DNA-Based Assays for Rapid Identification of Staphylococcus aureus. J. Clin. Microbiol. 36: 618-623 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»