This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gordillo, M E Articles by Murray, B E Search for Related Content PubMed PubMed Citation Articles by Gordillo, M E Articles by Murray, B E

Comparison of ribotyping and pulsed-field gel electrophoresis for subspecies differentiation of strains of Enterococcus faecalis.

Department of Internal Medicine, University of Texas Medical School, Houston 77030.

ABSTRACT

Hybridization of EcoRI- and HindIII-digested chromosomal DNAs from 41 isolates of Enterococcus faecalis with probes for rRNA genes was performed (ribotyping). The ability of ribotyping to distinguish strains at the subspecies level was compared with results previously determined by pulsed-field gel electrophoresis (PFGE). With EcoRI, seven ribopatterns (usually differing by only one band) were found, while PFGE had previously shown 25 clearly different patterns plus six related variants. Digestion with HindIII generated a few additional patterns but still failed to differentiate some strains that had very different PFGE patterns. Ribotyping with BscI has also been reported to be inadequate for subspecies strain differentiation (L. M. Hall, B. Duke, M. Guiney, and R. Williams, J. Clin. Microbiol. 30:915-919, 1992). Although ribotyping with other restriction endonucleases may perform better in distinguishing different strains, at present PFGE appears to be superior for strain differentiation.

This article has been cited by other articles:

• Singh, A., Goering, R. V., Simjee, S., Foley, S. L., Zervos, M. J. (2006). Application of Molecular Techniques to the Study of Hospital Infection. Clin. Microbiol. Rev. 19: 512-530 [Abstract] [Full Text]  
• Ruiz-Garbajosa, P., Bonten, M. J. M., Robinson, D. A., Top, J., Nallapareddy, S. R., Torres, C., Coque, T. M., Canton, R., Baquero, F., Murray, B. E., del Campo, R., Willems, R. J. L. (2006). Multilocus Sequence Typing Scheme for Enterococcus faecalis Reveals Hospital-Adapted Genetic Complexes in a Background of High Rates of Recombination.. J. Clin. Microbiol. 44: 2220-2228 [Abstract] [Full Text]  
• Nallapareddy, S. R., Wenxiang, H., Weinstock, G. M., Murray, B. E. (2005). Molecular Characterization of a Widespread, Pathogenic, and Antibiotic Resistance-Receptive Enterococcus faecalis Lineage and Dissemination of Its Putative Pathogenicity Island. J. Bacteriol. 187: 5709-5718 [Abstract] [Full Text]  
• Kuriyama, T., Williams, D. W., Patel, M., Lewis, M. A.O., Jenkins, L. E., Hill, D. W., Hosein, I. K. (2003). Molecular characterization of clinical and environmental isolates of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from a teaching hospital in Wales. J Med Microbiol 52: 821-827 [Abstract] [Full Text]  
• Duck, W. M., Steward, C. D., Banerjee, S. N., McGowan, J. E. Jr., Tenover, F. C. (2003). Optimization of Computer Software Settings Improves Accuracy of Pulsed-Field Gel Electrophoresis Macrorestriction Fragment Pattern Analysis. J. Clin. Microbiol. 41: 3035-3042 [Abstract] [Full Text]  
• Gelsomino, R., Vancanneyt, M., Cogan, T. M., Condon, S., Swings, J. (2002). Source of Enterococci in a Farmhouse Raw-Milk Cheese. Appl. Environ. Microbiol. 68: 3560-3565 [Abstract] [Full Text]  
• Homan, W. L., Tribe, D., Poznanski, S., Li, M., Hogg, G., Spalburg, E., van Embden, J. D. A., Willems, R. J. L. (2002). Multilocus Sequence Typing Scheme for Enterococcus faecium. J. Clin. Microbiol. 40: 1963-1971 [Abstract] [Full Text]  
• Price, C. S., Huynh, H., Paule, S., Hollis, R. J., Noskin, G. A., Pfaller, M. A., Peterson, L. R. (2002). Comparison of an Automated Ribotyping System to Restriction Endonuclease Analysis and Pulsed-Field Gel Electrophoresis for Differentiating Vancomycin-Resistant Enterococcus faecium Isolates. J. Clin. Microbiol. 40: 1858-1861 [Abstract] [Full Text]  
• Nallapareddy, S. R., Duh, R.-W., Singh, K. V., Murray, B. E. (2002). Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 40: 868-876 [Abstract] [Full Text]  
• Vancanneyt, M., Lombardi, A., Andrighetto, C., Knijff, E., Torriani, S., Bjorkroth, K. J., Franz, C. M. A. P., Foulquie Moreno, M. R., Revets, H., De Vuyst, L., Swings, J., Kersters, K., Dellaglio, F., Holzapfel, W. H. (2002). Intraspecies Genomic Groups in Enterococcus faecium and Their Correlation with Origin and Pathogenicity. Appl. Environ. Microbiol. 68: 1381-1391 [Abstract] [Full Text]  
• Sandoe, J. A. T., Witherden, I. R., Settle, C. (2001). Vertebral Osteomyelitis Caused by Enterococcus raffinosus. J. Clin. Microbiol. 39: 1678-1679 [Abstract] [Full Text]  
• Holzapfel, W. H, Haberer, P., Geisen, R., Bjorkroth, J., Schillinger, U. (2001). Taxonomy and important features of probiotic microorganisms in food and nutrition. Am. J. Clin. Nutr. 73: 365S-373 [Abstract] [Full Text]  
• Turabelidze, D., Kotetishvili, M., Kreger, A., Morris, J. G. Jr., Sulakvelidze, A. (2000). Improved Pulsed-Field Gel Electrophoresis for Typing Vancomycin-Resistant Enterococci. J. Clin. Microbiol. 38: 4242-4245 [Abstract] [Full Text]  
• Nallapareddy, S. R., Singh, K. V., Duh, R.-W., Weinstock, G. M., Murray, B. E. (2000). Diversity of ace, a Gene Encoding a Microbial Surface Component Recognizing Adhesive Matrix Molecules, from Different Strains of Enterococcus faecalis and Evidence for Production of Ace during Human Infections. Infect. Immun. 68: 5210-5217 [Abstract] [Full Text]  
• Bartie, K. L., Wilson, M. J., Williams, D. W., Lewis, M. A. O. (2000). Macrorestriction Fingerprinting of "Streptococcus milleri" Group Bacteria by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 38: 2141-2149 [Abstract] [Full Text]  
• Hammerum, A. M., Fussing, V., Aarestrup, F. M., Wegener, H. C. (2000). Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis. J Antimicrob Chemother 45: 677-680 [Abstract] [Full Text]  
• Morrison, D., Woodford, N., Barrett, S. P., Sisson, P., Cookson, B. D. (1999). DNA Banding Pattern Polymorphism in Vancomycin-Resistant Enterococcus faecium and Criteria for Defining Strains. J. Clin. Microbiol. 37: 1084-1091 [Abstract] [Full Text]  
• Savor, C., Pfaller, M. A., Kruszynski, J. A., Hollis, R. J., Noskin, G. A., Peterson, L. R. (1998). Comparison of Genomic Methods for Differentiating Strains of Enterococcus faecium: Assessment Using Clinical Epidemiologic Data. J. Clin. Microbiol. 36: 3327-3331 [Abstract] [Full Text]  
• Malathum, K, Singh, K., Weinstock, G., Murray, B. (1998). Repetitive sequence-based PCR versus pulsed-field gel electrophoresis for typing of Enterococcus faecalis at the subspecies level [In Process Citation]. J. Clin. Microbiol. 36: 211-215 [Abstract] [Full Text]