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J Clin Microbiol. 1993 June; 31(6): 1612-1614

Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.

K Subramanian, B Lupiani, F M Hetrick and S K Samal

College of Veterinary Medicine, University of Maryland, College Park 20742.

ABSTRACT

A nucleic acid hybridization assay was developed to rapidly detect small quantities of aquareovirus RNAs in infected cells and organs. Cloned cDNA copies were synthesized from the genomic RNA of the SBR strain of aquareovirus. By using cloned cDNA probes, aquareovirus RNAs were detected in spleen and kidney tissues of experimentally infected fish.

J Clin Microbiol. 1993 June; 31(6): 1612-1614






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1993 by the American Society for Microbiology. All rights reserved.