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Comparative evaluation of AccuProbe culture identification test for Neisseria gonorrhoeae and other rapid methods.

Scottish Gonococcal Reference Laboratory, Department of Medical Microbiology, Edinburgh University Medical School, Scotland.

ABSTRACT

The AccuProbe chemiluminescent culture identification test for Neisseria gonorrhoeae (Gen-Probe Inc., San Diego, Calif.) was assessed in a comparative evaluation with other rapid methods by using 269 isolates of oxidase-positive, gram-negative diplococci. Chemiluminescence was read with a PAL luminometer, and results were expressed as PAL light units (PLUs): the cutoff value for a positive identification was 1,500 PLUs. All 200 isolates of gonococci confirmed by carbohydrate utilization and serotyped with monoclonal antibodies were identified correctly by AccuProbe on initial testing. The API Quadferm system (Bio Merieux, Marcy l'Etoile, France) identified 95% (n = 190) of the gonococci correctly on initial testing and 99.5% (n = 199) on repeat testing, while the Phadebact Monoclonal GC test (Kara Bio Diagnostics AB, Huddinge, Sweden) identified 95.5% (n = 191) of the gonococci on both initial and repeat testing; 8 of the Phadebact-negative isolates were all of the same rare serovar (serovar 1B-17). The mean PLU for the gonococcal isolates was 9,014 (range 2,264 to 10,845) compared with a mean of 51 (range, 8 to 109) for the 69 nongonococcal isolates. We conclude that the AccuProbe culture confirmation test provides a rapid and accurate objective means of identifying cultured N. gonorrhoeae isolates.

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• Aslanzadeh, J., Jones, M. (2002). Comparison of M4 and M4RT Media for Transporting Cervical Swab Samples for PCR Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Annals of Clinical & Laboratory Science 32: 61-64 [Abstract] [Full Text]