This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bale, J F Articles by Murph, J R Search for Related Content PubMed PubMed Citation Articles by Bale, J F, Jr Articles by Murph, J R

Analysis of acquired human cytomegalovirus infections by polymerase chain reaction.

Department of Pediatrics, University of Iowa College of Medicine, Iowa City 52242.

ABSTRACT

We used the polymerase chain reaction and primers corresponding to three regions of the human cytomegalovirus (HCMV) genome to study HCMVs isolated from 16 children attending a single day-care center and the father of two children in the same center. When we analyzed isolates with primers for the pp65 and major immediate-early genes, we observed nearly uniform amplification yielding products of predicted sizes. By contrast, primers for the a sequence demonstrated variability among HCMV strains, supporting the use of these primers as an epidemiologic tool. Analysis of a-sequence products from two isolates demonstrated 50 to 70% nucleotide homology with the a sequence of HCMV Towne strain DNA. We observed 95% nucleotide homology for the two a-sequence products derived from the father-child pair. Analysis of day-care center isolates indicated that two children excreted two distinct HCMV strains during the study interval.

This article has been cited by other articles:

• Stranska, R., Schuurman, R., Toet, M., Verboon-Maciolek, M., de Vries, L. S., van Loon, A. M. (2006). Application of UL144 Molecular Typing To Determine Epidemiology of Cytomegalovirus Infections in Preterm Infants.. J. Clin. Microbiol. 44: 1108-1110 [Abstract] [Full Text]  
• Pignatelli, S., Dal Monte, P., Landini, M. P. (2001). gpUL73 (gN) genomic variants of human cytomegalovirus isolates are clustered into four distinct genotypes. J. Gen. Virol. 82: 2777-2784 [Abstract] [Full Text]  
• Walker, A., Petheram, S. J., Ballard, L., Murph, J. R., Demmler, G. J., Bale, J. F. Jr. (2001). Characterization of Human Cytomegalovirus Strains by Analysis of Short Tandem Repeat Polymorphisms. J. Clin. Microbiol. 39: 2219-2226 [Abstract] [Full Text]  
• Bale, J. F. Jr, Zimmerman, B., Dawson, J. D., Souza, I. E., Petheram, S. J., Murph, J. R. (1999). Cytomegalovirus Transmission in Child Care Homes. Arch Pediatr Adolesc Med 153: 75-79 [Abstract] [Full Text]