This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Switchenko, A C Articles by Ullman, E F Search for Related Content PubMed PubMed Citation Articles by Switchenko, A C Articles by Ullman, E F

An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species.

Research Department, Syva Company, Palo Alto, California 94304.

ABSTRACT

An automated enzymatic method was developed for the measurement of D-arabinitol in human serum. The assay is based on a novel, highly specific D-arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH produced is used in a second reaction to reduce p-iodonitrotetrazolium violet (INT) to INT-formazan, which is measured spectrophotometrically. The entire reaction sequence can be performed automatically on a COBAS MIRA-S clinical chemistry analyzer (Roche Diagnostic Systems, Inc., Montclair, N.J.). Replicate analyses of human sera supplemented with D-arabinitol over a concentration range of 0 to 40 microM demonstrated that the pentitol could be measured with an accuracy of +/- 7% and a precision (standard deviation) of +/- 0.4 microM. Serum D-arabinitol measurements correlated with those determined by gas chromatography (r = 0.94). The enzymatic method is unaffected by L-arabinitol, D-mannitol, or other polyols commonly found in human serum. Any of 17 therapeutic drugs potentially present in serum did not significantly influence assay performance. Data illustrating the application of the assay in patients for possible diagnosis of invasive candidiasis and the monitoring of therapeutic intervention are presented. The automated assay described here was developed to facilitate the investigation of D-arabinitol as a serum marker for invasive Candida infections.

This article has been cited by other articles:

• Yeo, S. F., Huie, S., Sofair, A. N., Campbell, S., Durante, A., Wong, B. (2006). Measurement of Serum D-Arabinitol/Creatinine Ratios for Initial Diagnosis and for Predicting Outcome in an Unselected, Population-Based Sample of Patients with Candida Fungemia. J. Clin. Microbiol. 44: 3894-3899 [Abstract] [Full Text]  
• Sendid, B., Caillot, D., Baccouch-Humbert, B., Klingspor, L., Grandjean, M., Bonnin, A., Poulain, D. (2003). Contribution of the Platelia Candida-Specific Antibody and Antigen Tests to Early Diagnosis of Systemic Candida tropicalis Infection in Neutropenic Adults. J. Clin. Microbiol. 41: 4551-4558 [Abstract] [Full Text]  
• Yeo, S. F., Wong, B. (2002). Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections. Clin. Microbiol. Rev. 15: 465-484 [Abstract] [Full Text]  
• SENDID, B., POIROT, J. L., TABOURET, M., BONNIN, A., CAILLOT, D., CAMUS, D., POULAIN, D. (2002). Combined detection of mannanaemia and anti-mannan antibodies as a strategy for the diagnosis of systemic infection caused by pathogenic Candida species. J Med Microbiol 51: 433-442 [Abstract] [Full Text]  
• Yeo, S. F., Zhang, Y., Schafer, D., Campbell, S., Wong, B. (2000). A Rapid, Automated Enzymatic Fluorometric Assay for Determination of D-Arabinitol in Serum. J. Clin. Microbiol. 38: 1439-1443 [Abstract] [Full Text]  
• Sendid, B., Tabouret, M., Poirot, J. L., Mathieu, D., Fruit, J., Poulain, D. (1999). New Enzyme Immunoassays for Sensitive Detection of Circulating Candida albicans Mannan and Antimannan Antibodies: Useful Combined Test for Diagnosis of Systemic Candidiasis. J. Clin. Microbiol. 37: 1510-1517 [Abstract] [Full Text]