This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lennon, P A Articles by Lee, S B Search for Related Content PubMed PubMed Citation Articles by Lennon, P A Articles by Lee, S B

Ribosomal DNA internal transcribed spacer analysis supports synonomy of Scedosporium inflatum and Lomentospora prolificans.

Department of Biological Sciences, University of Northern Colorado, Greeley 80639.

ABSTRACT

Scedosporium inflatum is a dematiaceous opportunistic pathogen originally described by D. Malloch and I.F. Salkin (Mycotaxon 21:247-255, 1984). However, E. Gueho and G. S. De Hoog (J. Mycol. Med. 118:3-9, 1991) recently suggested reducing this mold to synonomy with Lomentospora prolificans on the basis of their similar morphological and molecular characteristics. We have investigated the ribosomal DNA internal transcribed spacers (ITS), i.e., ITS I and ITS II, of 18 isolates, including these two fungi and a closely related pathogen, Scedosporium apiospermum, and its telemorph, Pseudallescheria boydii. Identical ITS restriction fragment length polymorphisms were found in eight isolates of S. inflatum and L. prolificans. These results support Gueho and De Hoog's proposal to combine S. inflatum and L. prolificans into the binomial Scedosporium prolificans. However, the ITS I sequence of S. apiospermum and the ITS restriction fragment length polymorphisms of S. apiospermum and P. boydii were found to be significantly different from those of S. inflatum and L. prolificans. The ITS restriction pattern differences may be valuable in clinical settings for distinguishing these fungi.

This article has been cited by other articles:

• Cortez, K. J., Roilides, E., Quiroz-Telles, F., Meletiadis, J., Antachopoulos, C., Knudsen, T., Buchanan, W., Milanovich, J., Sutton, D. A., Fothergill, A., Rinaldi, M. G., Shea, Y. R., Zaoutis, T., Kottilil, S., Walsh, T. J. (2008). Infections Caused by Scedosporium spp.. Clin. Microbiol. Rev. 21: 157-197 [Abstract] [Full Text]  
• Defontaine, A., Zouhair, R., Cimon, B., Carrere, J., Bailly, E., Symoens, F., Diouri, M., Hallet, J.-N., Bouchara, J.-P. (2002). Genotyping Study of Scedosporium apiospermum Isolates from Patients with Cystic Fibrosis. J. Clin. Microbiol. 40: 2108-2114 [Abstract] [Full Text]  
• Rainer, J., de Hoog, G. S., Wedde, M., Gräser, Y., Gilges, S. (2000). Molecular Variability of Pseudallescheria boydii, a Neurotropic Opportunist. J. Clin. Microbiol. 38: 3267-3273 [Abstract] [Full Text]