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J Clin Microbiol. 1994 October; 32(10): 2620-2621

Discriminatory genomic fingerprinting of Legionella pneumophila by pulsed-field electrophoresis.

W M Johnson, K Bernard, T J Marrie and S D Tyler

Laboratory Centre for Disease Control, Health Canada Tunney's Pasture, Ottawa, Ontario.

ABSTRACT

Eight strains of Legionella pneumophila were used to optimize cleavage of DNA with BssHII, SalI, or SpeI and separation by pulsed-field electrophoresis. Isolates from a community outbreak involving a contaminated hot tub were genomically identical. Cleavage patterns were distinctly different for unrelated environmental and nosocomial strains from a single hospital.

J Clin Microbiol. 1994 October; 32(10): 2620-2621




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