This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Soini, H Articles by Viljanen, M K Search for Related Content PubMed PubMed Citation Articles by Soini, H Articles by Viljanen, M K

Identification of mycobacteria by PCR-based sequence determination of the 32-kilodalton protein gene.

National Public Health Institute, Department in Turku, Finland.

ABSTRACT

In this study, a part of the nucleotide sequence of the mycobacterial 32-kDa protein gene was determined by PCR-based sequencing. A total of 24 mycobacterial strains, representing 10 species, were studied. Sequences of all tested members of the Mycobacterium tuberculosis complex were identical to each other and to the previously published sequence of M. tuberculosis H37Rv. The sequences of M. avium and M. intracellulare were different from each other. MAIX strains, identified with the Gen-Probe MAIX test, had sequences identical to each other but clearly different from those of M. avium and M. intracellulare. Each of the other mycobacterial species investigated, i.e., M. kansasii, M. gastri, M. gordonae, and M. malmoense, had a unique species-specific sequence. These results demonstrate that there is variation in the nucleotide sequence of the 32-kDa protein gene among different mycobacterial species. Thus, we propose that this gene can be used for PCR-based identification of mycobacteria.

This article has been cited by other articles:

• Gomila, M., Ramirez, A., Lalucat, J. (2007). Diversity of Environmental Mycobacterium Isolates from Hemodialysis Water as Shown by a Multigene Sequencing Approach. Appl. Environ. Microbiol. 73: 3787-3797 [Abstract] [Full Text]  
• Williams, K. J., Ling, C. L., Jenkins, C., Gillespie, S. H., McHugh, T. D. (2007). A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory. J Med Microbiol 56: 598-602 [Abstract] [Full Text]  
• Palomino, J. C. (2005). Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. Eur Respir J 26: 339-350 [Abstract] [Full Text]  
• O'Rourke, J. L., Solnick, J. V., Neilan, B. A., Seidel, K., Hayter, R., Hansen, L. M., Lee, A. (2004). Description of 'Candidatus Helicobacter heilmannii' based on DNA sequence analysis of 16S rRNA and urease genes. Int. J. Syst. Evol. Microbiol. 54: 2203-2211 [Abstract] [Full Text]  
• Turenne, C. Y., Thibert, L., Williams, K., Burdz, T. V., Cook, V. J., Wolfe, J. N., Cockcroft, D. W., Kabani, A. (2004). Mycobacterium saskatchewanense sp. nov., a novel slowly growing scotochromogenic species from human clinical isolates related to Mycobacterium interjectum and Accuprobe-positive for Mycobacterium avium complex. Int. J. Syst. Evol. Microbiol. 54: 659-667 [Abstract] [Full Text]  
• Cook, A. E., Meyers, P. R. (2003). Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns. Int. J. Syst. Evol. Microbiol. 53: 1907-1915 [Abstract] [Full Text]  
• Lee, H., Bang, H.-E., Bai, G.-H., Cho, S.-N. (2003). Novel Polymorphic Region of the rpoB Gene Containing Mycobacterium Species-Specific Sequences and Its Use in Identification of Mycobacteria. J. Clin. Microbiol. 41: 2213-2218 [Abstract] [Full Text]  
• Dauendorffer, J.-N., Guillemin, I., Aubry, A., Truffot-Pernot, C., Sougakoff, W., Jarlier, V., Cambau, E. (2003). Identification of Mycobacterial Species by PCR Sequencing of Quinolone Resistance-Determining Regions of DNA Gyrase Genes. J. Clin. Microbiol. 41: 1311-1315 [Abstract] [Full Text]  
• Makinen, J., Sarkola, A., Marjamaki, M., Viljanen, M. K., Soini, H. (2002). Evaluation of GenoType and LiPA MYCOBACTERIA Assays for Identification of Finnish Mycobacterial Isolates. J. Clin. Microbiol. 40: 3478-3481 [Abstract] [Full Text]  
• Appleyard, G. D., Clark, E. G. (2002). Histologic and Genotypic Characterization of a Novel Mycobacterium Species Found in Three Cats. J. Clin. Microbiol. 40: 2425-2430 [Abstract] [Full Text]  
• Menendez, M. C., Palenque, E., Navarro, M. C., Nunez, M. C., Rebollo, M. J., Garcia, M. J. (2001). Characterization of a Mycobacterium intracellulare Variant Strain by Molecular Techniques. J. Clin. Microbiol. 39: 4241-4246 [Abstract] [Full Text]  
• Suffys, P. N., da Silva Rocha, A., de Oliveira, M., Dias Campos, C. E., Werneck Barreto, A. M., Portaels, F., Rigouts, L., Wouters, G., Jannes, G., van Reybroeck, G., Mijs, W., Vanderborght, B. (2001). Rapid Identification of Mycobacteria to the Species Level Using INNO-LiPA Mycobacteria, a Reverse Hybridization Assay. J. Clin. Microbiol. 39: 4477-4482 [Abstract] [Full Text]  
• Soini, H., Musser, J. M. (2001). Molecular Diagnosis of Mycobacteria. Clin. Chem. 47: 809-814 [Abstract] [Full Text]  
• Lee, H., Park, H.-J., Cho, S.-N., Bai, G.-H., Kim, S.-J. (2000). Species Identification of Mycobacteria by PCR-Restriction Fragment Length Polymorphism of the rpoB Gene. J. Clin. Microbiol. 38: 2966-2971 [Abstract] [Full Text]  
• Kasai, H., Ezaki, T., Harayama, S. (2000). Differentiation of Phylogenetically Related Slowly Growing Mycobacteria by Their gyrB Sequences. J. Clin. Microbiol. 38: 301-308 [Abstract] [Full Text]  
• Junttila, J., Peltomaa, M., Soini, H., Marjamäki, M., Viljanen, M. K. (1999). Prevalence of Borrelia burgdorferi in Ixodes ricinus Ticks in Urban Recreational Areas of Helsinki. J. Clin. Microbiol. 37: 1361-1365 [Abstract] [Full Text]  
• Kauppinen, J., Mäntyjärvi, R., Katila, M.-L. (1999). Mycobacterium malmoense-Specific Nested PCR Based on a Conserved Sequence Detected in Random Amplified Polymorphic DNA Fingerprints. J. Clin. Microbiol. 37: 1454-1458 [Abstract] [Full Text]  
• Richter, E., Niemann, S., Rüsch-Gerdes, S., Hoffner, S. (1999). Identification of Mycobacterium kansasii by Using a DNA Probe (AccuProbe) and Molecular Techniques. J. Clin. Microbiol. 37: 964-970 [Abstract] [Full Text]  
• Seppälä, H., Skurnik, M., Soini, H., Roberts, M. C., Huovinen, P. (1998). A Novel Erythromycin Resistance Methylase Gene (ermTR) in Streptococcus pyogenes. Antimicrob. Agents Chemother. 42: 257-262 [Abstract] [Full Text]  
• Hellwage, J., Meri, T., Heikkila, T., Alitalo, A., Panelius, J., Lahdenne, P., Seppala, I. J. T., Meri, S. (2001). The Complement Regulator Factor H Binds to the Surface Protein OspE of Borrelia burgdorferi. J. Biol. Chem. 276: 8427-8435 [Abstract] [Full Text]