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J Clin Microbiol. 1994 February; 32(2): 473-476

Variation of DNA sequence in immediate-early gene of human herpesvirus 6 and variant identification by PCR.

T Yamamoto, T Mukai, K Kondo and K Yamanishi

Department of Virology, Osaka University, Japan.

ABSTRACT

The complete nucleotide sequence of one of the immediate-early genes of human herpesvirus 6 variant B was determined and compared with that of variant A reported by Martin et al. (M.D. Martin, J. Nicholas, B. J. Thomson, C. Newman, and R. W. Honess, J. Virol. 65:5381-5390, 1991). While it was reported that two open reading frames exist in this region of variant A, only one open reading frame was found in variant B and the putative coding region of variant B was 2,679 nucleotides long. Furthermore, two additive regions of 108 and 228 bp were found in variant B. Primers covering one of these regions deleted in variant A were synthesized and used for PCR amplification. Twelve isolates from patients were clearly classified into variants A and B by PCR amplification with these primers. All isolates from patients with exanthem subitum were variant B.

J Clin Microbiol. 1994 February; 32(2): 473-476




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