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J Clin Microbiol. 1994 March; 32(3): 832-834

Factors influencing detection of quantitative cytomegalovirus antigenemia.

M Boeckh, P M Woogerd, T Stevens-Ayers, C G Ray and R A Bowden

Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

ABSTRACT

Of 20 blood specimens testing positive for cytomegalovirus antigen after immediate processing, 19 (95%) remained positive when kept at room temperature for 24 h before processing. Quantitative antigenemia decreased by an average of 44% after storage. Compared with acetone fixation, formaldehyde fixation showed improved readability, fewer artifacts, and a higher degree of sensitivity.

J Clin Microbiol. 1994 March; 32(3): 832-834




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1994 by the American Society for Microbiology. All rights reserved.