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J Clin Microbiol. 1994 April; 32(4): 1074-1075

Modified technique for efficient detection of microsporidia.

McGill University Centre for Tropical Diseases, Montreal General Hospital, Canada.

ABSTRACT

Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.

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