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J Clin Microbiol. 1994 May; 32(5): 1246-1248

Direct detection of Vibrio cholerae in stool samples.

P Varela, G D Pollevick, M Rivas, I Chinen, N Binsztein, A C Frasch and R A Ugalde

Instituto Nacional de Microbiología Carlos G Malbrán, Buenos Aires, Argentina.

ABSTRACT

A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive PCRs, the second one with a nested primer, achieved the desired sensitivity. Comparison of the results obtained from stool swab samples processed by the two-step PCR and by an enzyme-linked immunosorbent assay using GM1 as the capture molecule showed that the former is more sensitive and gave positive results even when V. cholerae was not culturable or dead.

J Clin Microbiol. 1994 May; 32(5): 1246-1248




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