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J Clin Microbiol. 1994 May; 32(5): 1349-1353

Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions.

J T Saliki, J A House, C A Mebus and E J Dubovi

Foreign Animal Disease Diagnostic Laboratory, U.S. Department of Agriculture, Plum Island, Greenport, New York 11944.

ABSTRACT

A monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed for specific detection of peste des petits ruminants virus. Compared with virus isolation in Vero cell cultures using 89 paired tissue and secretion samples from six experimentally infected goats, S-ELISA was significantly more sensitive (71.9% versus 65.2%; P < 0.05). The S-ELISA is a suitable alternative to virus isolation.


J Clin Microbiol. 1994 May; 32(5): 1349-1353







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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1994 by the American Society for Microbiology. All rights reserved.