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Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing.

Nosocomial Pathogens Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.

ABSTRACT

An arbitrarily primed PCR (AP-PCR) assay was used to type Clostridium difficile isolates from a hospital outbreak of antibiotic-associated diarrhea. Forty-one isolates were separated into nine groups, with 66% falling into one group; no other group contained more than 10%. Comparison of AP-PCR grouping with that when the immunoblot technique was used showed agreement for 33 of 34 isolates typed by both techniques, and AP-PCR grouped seven isolates that were not typeable by immunoblotting.

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