This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pfaller, M A Articles by Steele-Moore, L Search for Related Content PubMed PubMed Citation Articles by Pfaller, M A Articles by Steele-Moore, L

Next Article 

J Clin Microbiol. 1994 July; 32(7): 1625-1628

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates.

Oregon Health Sciences University, Portland.

ABSTRACT

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

This article has been cited by other articles:

• Pfaller, M. A., Espinel-Ingroff, A., Jones, R. N. (2004). Clinical Evaluation of the Sensititre YeastOne Colorimetric Antifungal Plate for Antifungal Susceptibility Testing of the New Triazoles Voriconazole, Posaconazole, and Ravuconazole. J. Clin. Microbiol. 42: 4577-4580 [Abstract] [Full Text]  
• Ghannoum, M. A., Chaturvedi, V., Espinel-Ingroff, A., Pfaller, M. A., Rinaldi, M. G., Lee-Yang, W., Warnock, D. W. (2004). Intra- and Interlaboratory Study of a Method for Testing the Antifungal Susceptibilities of Dermatophytes. J. Clin. Microbiol. 42: 2977-2979 [Abstract] [Full Text]  
• Linares, M. J., Charriel, G., Solis, F., Casal, M. (2004). Comparison of Two Microdilution Methods for Testing Susceptibility of Candida spp. to Voriconazole. J. Clin. Microbiol. 42: 899-902 [Abstract] [Full Text]  
• Meletiadis, J., Mouton, J. W., Meis, J. F. G. M., Bouman, B. A., Verweij, P. E. (2002). Comparison of the Etest and the Sensititre Colorimetric Methods with the NCCLS Proposed Standard for Antifungal Susceptibility Testing of Aspergillus Species. J. Clin. Microbiol. 40: 2876-2885 [Abstract] [Full Text]  
• Pujol, I., Capilla, J., Fernandez-Torres, B., Ortoneda, M., Guarro, J. (2002). Use of the Sensititre Colorimetric Microdilution Panel for Antifungal Susceptibility Testing of Dermatophytes. J. Clin. Microbiol. 40: 2618-2621 [Abstract] [Full Text]  
• Meletiadis, J., Meis, J. F. G. M., Mouton, J. W., Donnelly, J. P., Verweij, P. E. (2000). Comparison of NCCLS and 3-(4,5-Dimethyl-2-Thiazyl)-2,5-Diphenyl-2H-Tetrazolium Bromide (MTT) Methods of In Vitro Susceptibility Testing of Filamentous Fungi and Development of a New Simplified Method. J. Clin. Microbiol. 38: 2949-2954 [Abstract] [Full Text]  
• Espinel-Ingroff, A., Pfaller, M., Messer, S. A., Knapp, C. C., Killian, S., Norris, H. A., Ghannoum, M. A. (1999). Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candida spp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms. J. Clin. Microbiol. 37: 591-595 [Abstract] [Full Text]  
• Pfaller, M. A., Messer, S. A., Karlsson, A., Bolmström, A. (1998). Evaluation of the Etest Method for Determining Fluconazole Susceptibilities of 402 Clinical Yeast Isolates by Using Three Different Agar Media. J. Clin. Microbiol. 36: 2586-2589 [Abstract] [Full Text]  
• Pfaller, M. A., Arikan, S., Lozano-Chiu, M., Chen, Y.-S., Coffman, S., Messer, S. A., Rennie, R., Sand, C., Heffner, T., Rex, J. H., Wang, J., Yamane, N. (1998). Clinical Evaluation of the ASTY Colorimetric Microdilution Panel for Antifungal Susceptibility Testing. J. Clin. Microbiol. 36: 2609-2612 [Abstract] [Full Text]  
• Hawser, S. P., Norris, H., Jessup, C. J., Ghannoum, M. A. (1998). Comparison of a 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Colorimetric Method with the Standardized National Committee for Clinical Laboratory Standards Method of Testing Clinical Yeast Isolates for Susceptibility to Antifungal Agents. J. Clin. Microbiol. 36: 1450-1452 [Abstract] [Full Text]