This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stanley, J Articles by Owen, R J Search for Related Content PubMed PubMed Citation Articles by Stanley, J Articles by Owen, R J

 Previous Article  |  Next Article 

J Clin Microbiol. 1994 July; 32(7): 1788-1794

Distinct genotypes of human and canine isolates of Campylobacter upsaliensis determined by 16S rRNA gene typing and plasmid profiling.

National Collection of Type Cultures, Central Public Health Laboratory, London, United Kingdom.

ABSTRACT

The utility of combined 16S rRNA (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within Campylobacter upsaliensis of human and canine origin was examined. Fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4.8 kb were observed. The copy number of the 16S rRNA gene was three in most strains of C. upsaliensis. Plasmids were found in almost 60% of the strains; ranging in size from 1.5 to 100 kb, they gave 15 distinct plasmid profiles. All isolates from humans contained one or more plasmids, as did strains isolated from dogs with sporadic diarrhea. The two commonest 16S ribotypes were divided into eight and nine subgroups by plasmid profiling. The genotyping of canine isolates from three veterinary surveys detected both multiple infections and reinfection of dogs. Except for one, each of the isolates from humans constituted a single and unique 16S ribotype, and these more frequently carried plasmids than did canine strains. Ribotypes of human strains were not found among canine isolates. These results suggest that host-specific genotypic differences may exist among strains of C. upsaliensis, for example, intraspecific clones or clone complexes pathogenic for humans.

This article has been cited by other articles:

• Damborg, P., Guardabassi, L., Pedersen, K., Kokotovic, B. (2008). Comparative Analysis of Human and Canine Campylobacter upsaliensis Isolates by Amplified Fragment Length Polymorphism. J. Clin. Microbiol. 46: 1504-1506 [Abstract] [Full Text]  
• Lentzsch, P., Rieksneuwohner, B., Wieler, L. H., Hotzel, H., Moser, I. (2004). High-Resolution Genotyping of Campylobacter upsaliensis Strains Originating from Three Continents. J. Clin. Microbiol. 42: 3441-3448 [Abstract] [Full Text]  
• Hald, B., Pedersen, K., Waino, M., Jorgensen, J. C., Madsen, M. (2004). Longitudinal Study of the Excretion Patterns of Thermophilic Campylobacter spp. in Young Pet Dogs in Denmark. J. Clin. Microbiol. 42: 2003-2012 [Abstract] [Full Text]  
• Fox, J G (2002). The non-H pylori helicobacters: their expanding role in gastrointestinal and systemic diseases. Gut 50: 273-283 [Abstract] [Full Text]  
• Moser, I., Rieksneuwohner, B., Lentzsch, P., Schwerk, P., Wieler, L. H. (2001). Genomic Heterogeneity and O-Antigenic Diversity of Campylobacter upsaliensis and Campylobacter helveticus Strains Isolated from Dogs and Cats in Germany. J. Clin. Microbiol. 39: 2548-2557 [Abstract] [Full Text]  
• Wassenaar, T. M., Newell, D. G. (2000). Genotyping of Campylobacter spp.. Appl. Environ. Microbiol. 66: 1-9 [Full Text]  
• Bourke, B., Chan, V. L., Sherman, P. (1998). Campylobacter upsaliensis: Waiting in the Wings. Clin. Microbiol. Rev. 11: 440-449 [Abstract] [Full Text]