This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Salomon, H Articles by Wainberg, M A Search for Related Content PubMed PubMed Citation Articles by Salomon, H Articles by Wainberg, M A

 Previous Article  |  Next Article 

J Clin Microbiol. 1994 August; 32(8): 2000-2002

Comparison of cord blood and peripheral blood mononuclear cells as targets for viral isolation and drug sensitivity studies involving human immunodeficiency virus type 1.

Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada.

ABSTRACT

We have shown that umbilical cord blood mononuclear cells (CBMC) are at least as sensitive as peripheral blood mononuclear cells (PBMC) for isolation of human immunodeficiency virus type 1 from the PBMC of infected individuals. Viral replication was more efficiently monitored by a p24 antigen capture assay than by a viral reverse transcriptase test, regardless of whether CBMC or PBMC were employed. We also found that CBMC and PBMC yielded similar results with regard to the susceptibility profiles of both wild-type and drug-resistant variants of human immunodeficiency virus type 1 for 3'-azido-3'-deoxythymidine, 2',3'-dideoxycytidine, and the (-) enantiomer of 2',3'-dideoxy-3'-thiacytidine. Finally, viruses isolated on CBMC could be routinely grown on PBMC and vice versa.

This article has been cited by other articles:

• Kajiwara, K., Kodama, E., Sakagami, Y., Naito, T., Matsuoka, M. (2008). Dual-Reporter Phenotypic Assay for Human Immunodeficiency Viruses. J. Clin. Microbiol. 46: 792-795 [Abstract] [Full Text]  
• Ntemgwa, M., Wainberg, M. A., Oliveira, M., Moisi, D., Lalonde, R., Micheli, V., Brenner, B. G. (2007). Variations in Reverse Transcriptase and RNase H Domain Mutations in Human Immunodeficiency Virus Type 1 Clinical Isolates Are Associated with Divergent Phenotypic Resistance to Zidovudine. Antimicrob. Agents Chemother. 51: 3861-3869 [Abstract] [Full Text]  
• Ntemgwa, M., Brenner, B. G., Oliveira, M., Moisi, D., Wainberg, M. A. (2007). Natural Polymorphisms in the Human Immunodeficiency Virus Type 2 Protease Can Accelerate Time to Development of Resistance to Protease Inhibitors. Antimicrob. Agents Chemother. 51: 604-610 [Abstract] [Full Text]  
• Petrella, M., Oliveira, M., Moisi, D., Detorio, M., Brenner, B. G., Wainberg, M. A. (2004). Differential Maintenance of the M184V Substitution in the Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Various Nucleoside Antiretroviral Agents in Tissue Culture. Antimicrob. Agents Chemother. 48: 4189-4194 [Abstract] [Full Text]  
• Quan, Y., Brenner, B. G., Oliveira, M., Wainberg, M. A. (2003). Lamivudine Can Exert a Modest Antiviral Effect against Human Immunodeficiency Virus Type 1 Containing the M184V Mutation. Antimicrob. Agents Chemother. 47: 747-754 [Abstract] [Full Text]  
• Kijak, G. H., Simon, V., Balfe, P., Vanderhoeven, J., Pampuro, S. E., Zala, C., Ochoa, C., Cahn, P., Markowitz, M., Salomon, H. (2002). Origin of Human Immunodeficiency Virus Type 1 Quasispecies Emerging after Antiretroviral Treatment Interruption in Patients with Therapeutic Failure. J. Virol. 76: 7000-7009 [Abstract] [Full Text]  
• Brenner, B. G., Routy, J.-P., Petrella, M., Moisi, D., Oliveira, M., Detorio, M., Spira, B., Essabag, V., Conway, B., Lalonde, R., Sekaly, R.-P., Wainberg, M. A. (2002). Persistence and Fitness of Multidrug-Resistant Human Immunodeficiency Virus Type 1 Acquired in Primary Infection. J. Virol. 76: 1753-1761 [Abstract] [Full Text]  
• Gu, Z., Wainberg, M. A., Nguyen-Ba, N., L'Heureux, L., de Muys, J.-M., Bowlin, T. L., Rando, R. F. (1999). Mechanism of Action and In Vitro Activity of 1',3'-Dioxolanylpurine Nucleoside Analogues against Sensitive and Drug-Resistant Human Immunodeficiency Virus Type 1 Variants. Antimicrob. Agents Chemother. 43: 2376-2382 [Abstract] [Full Text]