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J Clin Microbiol. 1994 August; 32(8): 2024-2027

Effects of enhancing agents on detection of cytomegalovirus in clinical specimens.

K St George and C R Rinaldo Jr

Department of Pathology, University of Pittsburgh Medical Center, Pennsylvania 15213.

ABSTRACT

Dimethyl sulfoxide, dexamethasone, and calcium were tested in combination for their enhancing effects on cytomegalovirus detection in shell vial cultures on 1,579 clinical specimens obtained primarily from adult solid-organ transplant recipients. Fluorescent-focus counts were elevated for the cytomegalovirus-positive urine specimens (P < 0.01) and throat washings (P < 0.05) but not for the tissue biopsy or blood samples. Epidermal growth factor also increased focus counts but provided no additional benefit when used in combination with the other agents. The triple-combination treatment did not increase the number of positive specimens identified.

J Clin Microbiol. 1994 August; 32(8): 2024-2027






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1994 by the American Society for Microbiology. All rights reserved.