This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wasieloski, L P Articles by Beaty, B J Search for Related Content PubMed PubMed Citation Articles by Wasieloski, L P, Jr Articles by Beaty, B J

Reverse transcription-PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation.

Department of Microbiology, Colorado State University, Fort Collins 80523.

ABSTRACT

A reverse transcription-PCR (RT-PCR) assay was developed and compared with enzyme immunoassay (EIA) and virus isolation for detecting LaCrosse virus (LAC) in mosquito pools. All three techniques were able to detect a single LAC-infected mosquito in a pool of 99 negative mosquitoes. Virus isolation was the most sensitive of the three techniques; it was possible to isolate virus immediately following intrathoracic inoculation of mosquitoes. RT-PCR was second in sensitivity; LAC RNA was detected 1 day postinfection. EIA detected LAC antigen 2 days postinfection. Additionally, RT-PCR and EIA were able to detect LAC RNA and protein, respectively, from mosquito samples which were subjected to seven freeze-thaw cycles, and RT-PCR was able to detect LAC RNA from mosquito samples which remained at room temperature for up to 7 days.

This article has been cited by other articles:

• Lambert, A. J., Nasci, R. S., Cropp, B. C., Martin, D. A., Rose, B. C., Russell, B. J., Lanciotti, R. S. (2005). Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA. J. Clin. Microbiol. 43: 1885-1889 [Abstract] [Full Text]  
• Borucki, M. K., Kempf, B. J., Blair, C. D., Beaty, B. J. (2001). The effect of mosquito passage on the La Crosse virus genotype. J. Gen. Virol. 82: 2919-2926 [Abstract] [Full Text]  
• Chandler, L. J., Borucki, M. K., Dobie, D. K., Wasieloski, L. P., Thompson, W. H., Gundersen, C. B., Case, K., Beaty, B. J. (1998). Characterization of La Crosse Virus RNA in Autopsied Central Nervous System Tissues. J. Clin. Microbiol. 36: 3332-3336 [Abstract] [Full Text]