This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Peek, R. M. Articles by Blaser, M. J. Search for Related Content PubMed PubMed Citation Articles by Peek, R. M., Jr Articles by Blaser, M. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 01 1995, 28-32, Vol 33, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Detection of Helicobacter pylori gene expression in human gastric mucosa

RM Peek Jr, GG Miller, KT Tham, GI Perez-Perez, TL Cover, JC Atherton, GD Dunn and MJ Blaser
Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2279.

Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy specimens and serum for anti-H. pylori immunoglobulin G serology were obtained from 42 patients. Biopsy specimens were studied by histology, culture, and reverse transcription PCR (RT-PCR). Oligonucleotide primers specific for H. pylori (16S rRNA, ureA, and cagA) were used to detect bacterial mRNA in gastric biopsy specimens. PCR was performed on DNA from corresponding H. pylori isolates to detect genomic 16S rRNA, ureA, and cagA. Of the 42 patients from whom clinical specimens were obtained, 25 were infected with H. pylori on the basis of both serology and histology or culture (i.e., tissue positive); 13 were negative by serology, histology, and culture; and 4 were positive by serology only. RT-PCR with 16S rRNA primers detected 24 of 25 tissue-positive and 0 of 17 tissue-negative patients (P < 0.001). RT-PCR with ureA primers detected 16 of 25 tissue-positive and 0 of 17 tissue-negative patients (P < 0.001). CagA mRNA was detected by RT-PCR in 14 of 25 gastric biopsy specimens in the tissue- positive group and in 0 of 17 gastric biopsy specimens in the tissue- negative group. PCR of genomic DNA for the presence of the cagA gene in the corresponding bacterial isolates correlated absolutely with cagA gene expression in gastric tissue. These results indicate that RT-PCR is a sensitive and specific method for the detection of the presence of H. pylori and the expression of H. pylori genes in human gastric tissue. Detection of H. pylori gene expression in vivo by this approach may contribute to improving the diagnosis and understanding the pathogenesis of H. pylori infections.

This article has been cited by other articles:

• Peek, R. M. Jr (2008). Review: Prevention of gastric cancer: When is treatment of Helicobacter pylori warranted?. Therapeutic Advances in Gastroenterology 1: 19-31 [Abstract]  
• Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
• Bhat, N., Gaensbauer, J., Peek, R. M., Bloch, K., Tham, K.-T., Blaser, M. J., Perez-Perez, G. (2005). Local and Systemic Immune and Inflammatory Responses to Helicobacter pylori Strains. CVI 12: 1393-1400 [Abstract] [Full Text]  
• Rocha, A. M. C., Rocha, G. A., Santos, A., de Oliveira, C. A., Queiroz, D. M. M. (2003). Accuracy of a Commercial Enzyme-Linked Immunosorbent Assay for CagA in Patients from Brazil with and without Gastric Carcinoma. J. Clin. Microbiol. 41: 447-448 [Abstract] [Full Text]  
• Cao, P., Cover, T. L. (2002). Two Different Families of hopQ Alleles in Helicobacter pylori. J. Clin. Microbiol. 40: 4504-4511 [Abstract] [Full Text]  
• Romero-Gallo, J., Perez-Perez, G. I., Novick, R. P., Kamath, P., Norbu, T., Blaser, M. J. (2002). Responses of Endoscopy Patients in Ladakh, India, to Helicobacter pylori Whole-Cell and CagA Antigens. CVI 9: 1313-1317 [Abstract] [Full Text]  
• Queiroz, D. M. M., Rocha, G. A., Oliveira, C. A. d., Rocha, A. M. C., Santos, A., Cabral, M. M. D. A., Nogueira, A. M. M. F. (2002). Role of Corpus Gastritis and cagA-Positive Helicobacter pylori Infection in Reflux Esophagitis. J. Clin. Microbiol. 40: 2849-2853 [Abstract] [Full Text]  
• Prazeres Magalhaes, P., de Magalhaes Queiroz, D. M., Campos Barbosa, D. V., Aguiar Rocha, G., Nogueira Mendes, E., Santos, A., Valle Correa, P. R., Camargos Rocha, A. M., Martins Teixeira, L., Affonso de Oliveira, C. (2002). Helicobacter pylori Primary Resistance to Metronidazole and Clarithromycin in Brazil. Antimicrob. Agents Chemother. 46: 2021-2023 [Abstract] [Full Text]  
• Nomura, A. M. Y., Perez-Perez, G. I., Lee, J., Stemmermann, G., Blaser, M. J. (2002). Relation between Helicobacter pylori cagA Status and Risk of Peptic Ulcer Disease. Am J Epidemiol 155: 1054-1059 [Abstract] [Full Text]  
• Israel, D. A., Salama, N., Krishna, U., Rieger, U. M., Atherton, J. C., Falkow, S., Peek, R. M. Jr. (2001). Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc. Natl. Acad. Sci. USA 10.1073/pnas.251551698v1 [Abstract] [Full Text]  
• Rota, C. A., Pereira-Lima, J. C., Blaya, C., Nardi, N. B. (2001). Consensus and Variable Region PCR Analysis of Helicobacter pylori 3' Region of cagA Gene in Isolates from Individuals with or without Peptic Ulcer. J. Clin. Microbiol. 39: 606-612 [Abstract] [Full Text]  
• Peek, R. M. Jr., van Doorn, L.-J., Donahue, J. P., Tham, K. T., Figueiredo, C., Blaser, M. J., Miller, G. G. (2000). Quantitative Detection of Helicobacter pylori Gene Expression In Vivo and Relationship to Gastric Pathology. Infect. Immun. 68: 5488-5495 [Abstract] [Full Text]  
• Yahav, J., Fradkin, A., Weisselberg, B., Diver-Haver, A., Shmuely, H., Jonas, A. (2000). Relevance of CagA Positivity to Clinical Course of Helicobacter pylori Infection in Children. J. Clin. Microbiol. 38: 3534-3537 [Abstract] [Full Text]  
• Yoshimura, T, Shimoyama, T, Tanaka, M, Sasaki, Y, Fukuda, S, Munakata, A (2000). Gastric mucosal inflammation and epithelial cell turnover are associated with gastric cancer in patients with Helicobacter pylori infection. J. Clin. Pathol. 53: 532-536 [Abstract] [Full Text]  
• MIZUKI, I., SHIMOYAMA, T., FUKUDA, S., LIU, Q., NAKAJI, S., MUNAKATA, A. (2000). Association of gastric epithelial apoptosis with the ability of Helicobacter pylori to induce a neutrophil oxidative burst. J Med Microbiol 49: 521-524 [Abstract] [Full Text]  
• Danielsson, D, Farmery, S M, Blomberg, B, Perry, S, Rautelin, H, Crabtree, J E (2000). Co-expression in Helicobacter pylori of cagA and non-opsonic neutrophil activation enhances the association with peptic ulcer disease. J. Clin. Pathol. 53: 318-321 [Abstract] [Full Text]  
• Gerhard, M., Lehn, N., Neumayer, N., Boren, T., Rad, R., Schepp, W., Miehlke, S., Classen, M., Prinz, C. (1999). Clinical relevance of the Helicobacter pylori gene for blood-group antigen-binding adhesin. Proc. Natl. Acad. Sci. USA 96: 12778-12783 [Abstract] [Full Text]  
• Gupta, R. A., Polk, D. B., Krishna, U., Israel, D. A., Yan, F., DuBois, R. N., Peek, R. M. Jr. (2001). Activation of Peroxisome Proliferator-activated Receptor gamma Suppresses Nuclear Factor kappa B-mediated Apoptosis Induced by Helicobacter pylori in Gastric Epithelial Cells. J. Biol. Chem. 276: 31059-31066 [Abstract] [Full Text]  
• Israel, D. A., Salama, N., Krishna, U., Rieger, U. M., Atherton, J. C., Falkow, S., Peek, R. M. Jr. (2001). Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc. Natl. Acad. Sci. USA 98: 14625-14630 [Abstract] [Full Text]