This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liebana, E. Articles by Dominguez, L. Search for Related Content PubMed PubMed Citation Articles by Liebana, E. Articles by Dominguez, L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Jan 1995, 33-36, Vol 33, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Simple and rapid detection of Mycobacterium tuberculosis complex organisms in bovine tissue samples by PCR

E Liebana, A Aranaz, A Mateos, M Vilafranca, E Gomez-Mampaso, JC Tercero, J Alemany, G Suarez, M Domingo and L Dominguez
Departamento de Patologia Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.

Mycobacterium bovis is a slowly growing microorganism, and confirmation of the diagnosis by conventional culture is a lengthy process. A simple, rapid method for the extraction of DNA from bovine tissue samples was developed and used in a PCR designed for the diagnosis of tuberculosis. Tissues from 81 cattle from tuberculosis-infected herds (group 1) and 19 cattle from tuberculosis-free herds (group 2) were tested in this PCR, and the results were compared with those of conventional culture. The PCR assay detected 71.4% of the culture- positive animals from group 1. Tissue from all animals in group 2 were negative in the PCR assay and by culture. The described method could be used as a rapid screening technique which would be complementary to culture of tissue specimens for the routine diagnosis of bovine tuberculosis. The PCR technique is much faster than culture and reduces the time for diagnosis from several months to 2 days. It also provides for the detection of M. bovis when rapidly growing Mycobacterium spp. are present in the sample and may be able to detect the presence of M. bovis in samples even when organisms have become nonviable.

This article has been cited by other articles:

• Taylor, M. J., Hughes, M. S., Skuce, R. A., Neill, S. D. (2001). Detection of Mycobacterium bovis in Bovine Clinical Specimens Using Real-Time Fluorescence and Fluorescence Resonance Energy Transfer Probe Rapid-Cycle PCR. J. Clin. Microbiol. 39: 1272-1278 [Abstract] [Full Text]  
• Coetsier, C., Vannuffel, P., Blondeel, N., Denef, J.-F., Cocito, C., Gala, J.-L. (2000). Duplex PCR for Differential Identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in Formalin- Fixed Paraffin-Embedded Tissues from Cattle. J. Clin. Microbiol. 38: 3048-3054 [Abstract] [Full Text]