This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Haynes, K. A. Articles by Cann, K. J. Search for Related Content PubMed PubMed Citation Articles by Haynes, K. A. Articles by Cann, K. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Jan 1995, 99-102, Vol 33, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Involvement of multiple Cryptococcus neoformans strains in a single episode of cryptococcosis and reinfection with novel strains in recurrent infection demonstrated by random amplification of polymorphic DNA and DNA fingerprinting

KA Haynes, DJ Sullivan, DC Coleman, JC Clarke, R Emilianus, C Atkinson and KJ Cann
Department of Medical Microbiology, Charing Cross and Westminster Medical School, London, United Kingdom.

We compared the abilities of random amplification of polymorphic DNA and DNA fingerprinting, with oligonucleotide probes, to type five pairs of Cryptococcus neoformans clinical isolates recovered from five separate human immunodeficiency virus-positive patients in London, England. The two techniques had comparable discriminatory abilities when applied to these isolates. A total of eight different isolate types were demonstrated in these patients. No isolate type was observed in more than one patient. Two of the isolate pairs recovered from single episodes of cryptococcosis within 1 day of each other were genotypically indistinguishable by both methods. The other three pairs of isolates were all distinguishable. One of these isolate pairs was obtained from a single episode of cryptococcosis, while the other two were obtained from recurrent infections. These results indicate that multiple strains of C. neoformans may be responsible for a single episode of cryptococcosis and that recurrent infection may occur as a result of reinfection with a novel strain.

This article has been cited by other articles:

• Lui, G., Lee, N., Ip, M., Choi, K.W., Tso, Y.K., Lam, E., Chau, S., Lai, R., Cockram, C.S. (2006). Cryptococcosis in apparently immunocompetent patients. QJM 99: 143-151 [Abstract] [Full Text]  
• Jain, N., Wickes, B. L., Keller, S. M., Fu, J., Casadevall, A., Jain, P., Ragan, M. A., Banerjee, U., Fries, B. C. (2005). Molecular Epidemiology of Clinical Cryptococcus neoformans Strains from India. J. Clin. Microbiol. 43: 5733-5742 [Abstract] [Full Text]  
• Mandal, P., Banerjee, U., Casadevall, A., Nosanchuk, J. D. (2005). Dual Infections with Pigmented and Albino Strains of Cryptococcus neoformans in Patients with or without Human Immunodeficiency Virus Infection in India. J. Clin. Microbiol. 43: 4766-4772 [Abstract] [Full Text]  
• Nielsen, K., Cox, G. M., Litvintseva, A. P., Mylonakis, E., Malliaris, S. D., Benjamin, D. K. Jr., Giles, S. S., Mitchell, T. G., Casadevall, A., Perfect, J. R., Heitman, J. (2005). Cryptococcus neoformans {alpha} Strains Preferentially Disseminate to the Central Nervous System during Coinfection. Infect. Immun. 73: 4922-4933 [Abstract] [Full Text]  
• Litvintseva, A. P., Kestenbaum, L., Vilgalys, R., Mitchell, T. G. (2005). Comparative Analysis of Environmental and Clinical Populations of Cryptococcus neoformans. J. Clin. Microbiol. 43: 556-564 [Abstract] [Full Text]  
• Sukroongreung, S., Lim, S., Tantimavanich, S., Eampokalap, B., Carter, D., Nilakul, C., Kulkeratiyut, S., Tansuphaswadikul, S. (2001). Phenotypic Switching and Genetic Diversity of Cryptococcus neoformans. J. Clin. Microbiol. 39: 2060-2064 [Abstract] [Full Text]  
• Bertout, S., Renaud, F., Swinne, D., Mallié, M., Bastide, J.-M. (1999). Genetic Multilocus Studies of Different Strains of Cryptococcus neoformans: Taxonomy and Genetic Structure. J. Clin. Microbiol. 37: 715-720 [Abstract] [Full Text]  
• Klepser, M. E., Pfaller, M. A. (1998). Variation in Electrophoretic Karyotype and Antifungal Susceptibility of Clinical Isolates of Cryptococcus neoformans at a University-Affiliated Teaching Hospital from 1987 to 1994. J. Clin. Microbiol. 36: 3653-3656 [Abstract] [Full Text]