This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smits, H. L. Articles by Ter Schegget, J. Search for Related Content PubMed PubMed Citation Articles by Smits, H. L. Articles by Ter Schegget, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 10 1995, 2631-2636, Vol 33, No. 10
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Intermethod variation in detection of human papillomavirus DNA in cervical smears

HL Smits, LJ Bollen, SP Tjong-A-Hung, J Vonk, J Van Der Velden, FJ Ten Kate, JA Kaan, BW Mol and J Ter Schegget
Department of Virology, University of Amsterdam, The Netherlands.

In order to investigate the reliability of detection of human papillomavirus (HPV) DNA in cervical smears, we have compared the performance of two HPV PCR systems, the CPI/IIG and MY09/11 primer- mediated PCRs and the Hybrid Capture System HPV DNA detection test (hybrid capture assay), in detecting HPV DNA in cervical smears. We also included in our study the MY09/11B PCR plus SHARP (solution hybridization assay for PCR products) Signal System. This SHARP Signal System was recently developed to detect MY09/11B-generated biotinylated PCR products. The detection rate of the hybrid capture assay was lower than those of the CPI/IIG and MY09/11 PCRs and the MY09/11B PCR plus SHARP Signal System. The detection rates of the CPI/IIG PCR and the MY09/11B PCR plus SHARP Signal System were similar and higher than that of the conventional MY09/11 PCR system. The agreement beyond chance of the PCR methods was nearly perfect (kappa value between 0.82 and 0.84). The agreement beyond chance of the hybrid capture assay and the PCR methods was fair to good (kappa value between 0.64 and 0.70). The systems detected HPV DNA in different but overlapping sets of smears. Our results indicate that each of the detection methods alone underestimates the prevalence of HPV.

This article has been cited by other articles:

• Gheit, T., Landi, S., Gemignani, F., Snijders, P. J. F., Vaccarella, S., Franceschi, S., Canzian, F., Tommasino, M. (2006). Development of a sensitive and specific assay combining multiplex PCR and DNA microarray primer extension to detect high-risk mucosal human papillomavirus types.. J. Clin. Microbiol. 44: 2025-2031 [Abstract] [Full Text]  
• van Ham, M. A. P. C., Bakkers, J. M. J. E., Harbers, G. K., Quint, W. G. V., Massuger, L. F. A. G., Melchers, W. J. G. (2005). Comparison of Two Commercial Assays for Detection of Human Papillomavirus (HPV) in Cervical Scrape Specimens: Validation of the Roche AMPLICOR HPV Test as a Means To Screen for HPV Genotypes Associated with a Higher Risk of Cervical Disorders. J. Clin. Microbiol. 43: 2662-2667 [Abstract] [Full Text]  
• Gharizadeh, B., Kaller, M., Nyren, P., Andersson, A., Uhlen, M., Lundeberg, J., Ahmadian, A. (2003). Viral and microbial genotyping by a combination of multiplex competitive hybridization and specific extension followed by hybridization to generic tag arrays. Nucleic Acids Res 31: e146-e146 [Abstract] [Full Text]  
• Kornegay, J. R., Roger, M., Davies, P. O., Shepard, A. P., Guerrero, N. A., Lloveras, B., Evans, D., Coutlee, F. (2003). International Proficiency Study of a Consensus L1 PCR Assay for the Detection and Typing of Human Papillomavirus DNA: Evaluation of Accuracy and Intralaboratory and Interlaboratory Agreement. J. Clin. Microbiol. 41: 1080-1086 [Abstract] [Full Text]  
• Kleter, B., van Doorn, L.-J., Schrauwen, L., Molijn, A., Sastrowijoto, S., ter Schegget, J., Lindeman, J., ter Harmsel, B., Burger, M., Quint, W. (1999). Development and Clinical Evaluation of a Highly Sensitive PCR-Reverse Hybridization Line Probe Assay for Detection and Identification of Anogenital Human Papillomavirus. J. Clin. Microbiol. 37: 2508-2517 [Abstract] [Full Text]  
• Poljak, M., Brencic, A., Seme, K., Vince, A., Marin, I. J. (1999). Comparative Evaluation of First- and Second-Generation Digene Hybrid Capture Assays for Detection of Human Papillomaviruses Associated with High or Intermediate Risk for Cervical Cancer. J. Clin. Microbiol. 37: 796-797 [Abstract] [Full Text]  
• Kleter, B., van Doorn, L.-J., ter Schegget, J., Schrauwen, L., van Krimpen, K., Burger, M., ter Harmsel, B., Quint, W. (1998). Novel Short-Fragment PCR Assay for Highly Sensitive Broad-Spectrum Detection of Anogenital Human Papillomaviruses. Am. J. Pathol. 153: 1731-1739 [Abstract] [Full Text]  
• Peyton, C. L., Schiffman, M., Lörincz, A. T., Hunt, W. C., Mielzynska, I., Bratti, C., Eaton, S., Hildesheim, A., Morera, L. A., Rodriguez, A. C., Herrero, R., Sherman, M. E., Wheeler, C. M. (1998). Comparison of PCR- and Hybrid Capture-Based Human Papillomavirus Detection Systems Using Multiple Cervical Specimen Collection Strategies. J. Clin. Microbiol. 36: 3248-3254 [Abstract] [Full Text]  
• Lorincz, A., Tang, Y.-W., Persing, D. H. (1998). Hybrid Capture. Clin. Chem. 44: 1363-1363 [Full Text]