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Journal of Clinical Microbiology, 10 1995, 2707-2709, Vol 33, No. 10
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Association between alpha-hemolysin production and HeLa cell-detaching activity in fecal isolates of Escherichia coli

LR Marques, CM Abe, PM Griffin and TA Gomes
Seccao de Bacteriologia, Instituto Adolfo Lutz, Sao Paulo, Brazil.

Escherichia coli isolates that cause detachment of cell monolayers during in vitro adherence assays (cell-detaching E. coli [CDEC]) were recently reported as a potential new group of enteropathogenic bacteria. In the present study, 269 E. coli isolates from feces of children 1 to 5 years of age were identified as CDEC in a detaching assay developed with HeLa cells. The great majority of these isolates were hemolytic within 3 h of growth on blood agar plates and hybridized with a DNA probe for alpha-hemolysin (93.7%), while most of the non- detaching isolates were hemolytic within 24 h (3.6%) or nonhemolytic (94.8%). E. coli isolates that produced alpha-hemolysin were found in 60 (30%) of 200 children with diarrhea and 47 (24%) of 200 age-matched controls. No statistical significance was found for the differences in alpha-hemolysin production among the matched pairs (P = 0.2). These data suggest that CDEC isolates are not associated with diarrhea in the population studied.

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