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Journal of Clinical Microbiology, 10 1995, 2777-2780, Vol 33, No. 10
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance

PS Eastman, E Boyer, L Mole, J Kolberg, M Urdea and M Holodniy
Chiron Corporation, Emeryville, California 94608, USA.

A nonisotopic hybridization assay for human immunodeficiency virus genotypic zidovudine resistance determination is described. Biotinylated PCR product was hybridized with enzyme-labeled probes for wild-type or resistant mutant sequences and detected colorimetrically or chemiluminescently in a microplate format. Changes in mutant-to-wild- type ratios allow for the monitoring of longitudinal patient samples.

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