This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Albert, M. J. Articles by Islam, M. S. Search for Related Content PubMed PubMed Citation Articles by Albert, M. J. Articles by Islam, M. S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Dec 1995, 3119-3123, Vol 33, No. 12
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Characterization of Aeromonas trota strains that cross-react with Vibrio cholerae O139 Bengal

MJ Albert, M Ansaruzzaman, T Shimada, A Rahman, NA Bhuiyan, S Nahar, F Qadri and MS Islam
Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh. albert%cholera@external.ait.ac.th.

It has previously been shown that Vibrio cholerae O139 Bengal shares antigens with V. cholerae serogroups O22 and O155. We detected six surface water isolates of Aeromonas trota that agglutinated in polyclonal antisera to V. cholerae O139 and V. cholerae O22 but not in antiserum to V. cholerae O155. On the basis of agglutinin-absorption studies, the antigenic relationship between the cross-reacting bacteria were found to be in an a,b-a,c fashion, where a is the common antigenic epitope and b and c are unique epitopes. The antigen sharing between A. trota strains and V. cholerae O139 was confirmed in immunoblot studies. However, A. trota strains did not react with two monoclonal antibodies specific for V. cholerae O139 and, consequently, tested negative in the Bengal SMART rapid diagnostic test for V. cholerae O139 which uses one of the monoclonal antibodies. A polyclonal antiserum to a cross- reacting A. trota strain cross-protected infant mice against cholera on challenge with virulent V. cholerae O139. All A. trota strains were cytotoxic for HeLa cells, positive for adherence to HEp-2 cells, and weakly invasive for HEp-2 cells; one strain was heat-stable toxin positive in the suckling mouse assay; however, all strains were negative for cholera toxin-like enterotoxin. Studies on bacteria that share somatic antigen with V. cholerae O139 may shed further light on the genesis of V. cholerae O139.

This article has been cited by other articles:

• Udden, S. M. N., Zahid, M. S. H., Biswas, K., Ahmad, Q. S., Cravioto, A., Nair, G. B., Mekalanos, J. J., Faruque, S. M. (2008). Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence. Proc. Natl. Acad. Sci. USA 105: 11951-11956 [Abstract] [Full Text]  
• Ansaruzzaman, M, Sultana, M., Talukder, K. A, Alam, K, Matsushita, S, Safa, A., Khajanchi, B. K, Dutta, D. K, Islam, Z., Albert, M J., Nair, G B., Sack, D. A (2005). Isolation and characterization of provisional serovar Shigella boydii E16553 from diarrhoeal patients in Bangladesh. J Med Microbiol 54: 477-480 [Abstract] [Full Text]  
• Bhuiyan, N. A., Qadri, F., Faruque, A. S. G., Malek, M. A, Salam, M. A., Nato, F., Fournier, J. M., Chanteau, S., Sack, D. A., Balakrish Nair, G. (2003). Use of Dipsticks for Rapid Diagnosis of Cholera Caused by Vibrio cholerae O1 and O139 from Rectal Swabs. J. Clin. Microbiol. 41: 3939-3941 [Abstract] [Full Text]  
• KUMAR, S., RAY, P., SINGH, H., GANGULY, N. K. (2001). Immunogenicity and protective role of an IgA reactive 31-kDa antigen of Vibrio cholerae O139. J Med Microbiol 50: 489-498 [Abstract] [Full Text]