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Journal of Clinical Microbiology, 02 1995, 331-335, Vol 33, No. 2
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Proposed quality control guidelines for antimicrobial susceptibility tests using tilmicosin

TR Shryock, DW White, CS Werner and JM Staples
Lilly Research Laboratories, Eli Lilly and Company, Greenfield, Indiana 46140.

Quality control guidelines for tilmicosin, a novel veterinary-use-only macrolide, were developed in a multi-laboratory study according to established National Committee for Clinical Laboratory Standards (NCCLS) procedures (M23-T2). Tilmicosin was incorporated into Sensititre plates for broth microdilution endpoint testing and into two lots of 15-micrograms disks for Kirby-Bauer agar disk diffusion testing. One common lot and five unique lots of Mueller-Hinton media were used. (Broth was cation adjusted, and agar was supplemented with 5% defibrinated sheep blood.) Bacteria used for reference strains included Pasteurella haemolytica 128K, Pasteurella multocida ATCC 43137, and Staphylococcus aureus ATCC 29213 (microdilution) and ATCC 25923 (disk). Replicate tests were conducted. Disk diffusion and broth microdilution quality control ranges are proposed.

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