This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kapur, V.
Right arrow Articles by Musser, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kapur, V.
Right arrow Articles by Musser, J. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Feb 1995, 376-380, Vol 33, No. 2
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Molecular population genetic analysis of Staphylococcus aureus recovered from cows

V Kapur, WM Sischo, RS Greer, TS Whittam and JM Musser
Department of Pathology, Baylor College of Medicine, Houston, Texas 77030.

Staphylococcus aureus is one of the most common causes of bovine mastitis. To estimate genetic relationships among S. aureus strains recovered from cows, 357 isolates from milk samples from worldwide localities were examined for electrophoretic variation at 13 metabolic- enzyme loci. Thirty-nine electrophoretic types which represented distinctive multilocus enzyme genotypes were identified, and nearly 90% of all isolates were assigned to one of eight clones. Genetic heterogeneity was found among organisms recovered from dairy herds from which multiple isolates were obtained, indicating that the S. aureus population in a single herd can be multiclonal. Although humans and cows shared 7 of the 39 S. aureus clones, each clone was predominantly associated with one of these host species. These results are consistent with the concept of host specialization among S. aureus clones and imply that successful transfer of bacteria between humans and cows is limited.


This article has been cited by other articles:

  • Ciftci, A., Onuk, E. E., Findik, A., Yildirim, T., Sogut, M. U. (2009). Molecular typing of Staphylococcus aureus strains from ovine mastitis by pulsed-field gel electrophoresis and polymerase chain reaction based on coagulase and protein A gene polymorphisms. jvdi 21: 849-853 [Abstract] [Full Text]  
  • Smyth, D. S., Feil, E. J., Meaney, W. J., Hartigan, P. J., Tollersrud, T., Fitzgerald, J. R., Enright, M. C., Smyth, C. J. (2009). Molecular genetic typing reveals further insights into the diversity of animal-associated Staphylococcus aureus. J Med Microbiol 58: 1343-1353 [Abstract] [Full Text]  
  • Collery, M. M., Smyth, D. S., Tumilty, J. J. G., Twohig, J. M., Smyth, C. J. (2009). Associations between enterotoxin gene cluster types egc1, egc2 and egc3, agr types, enterotoxin and enterotoxin-like gene profiles, and molecular typing characteristics of human nasal carriage and animal isolates of Staphylococcus aureus. J Med Microbiol 58: 13-25 [Abstract] [Full Text]  
  • Ben Zakour, N. L., Sturdevant, D. E., Even, S., Guinane, C. M., Barbey, C., Alves, P. D., Cochet, M.-F., Gautier, M., Otto, M., Fitzgerald, J. R., Le Loir, Y. (2008). Genome-Wide Analysis of Ruminant Staphylococcus aureus Reveals Diversification of the Core Genome. J. Bacteriol. 190: 6302-6317 [Abstract] [Full Text]  
  • Sung, J. M.-L., Lloyd, D. H., Lindsay, J. A. (2008). Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray. Microbiology 154: 1949-1959 [Abstract] [Full Text]  
  • Rabello, R. F., Moreira, B. M., Lopes, R. M. M., Teixeira, L. M., Riley, L. W., Castro, A. C. D. (2007). Multilocus sequence typing of Staphylococcus aureus isolates recovered from cows with mastitis in Brazilian dairy herds. J Med Microbiol 56: 1505-1511 [Abstract] [Full Text]  
  • Aires-de-Sousa, M., Parente, C. E. S. R., Vieira-da-Motta, O., Bonna, I. C. F., Silva, D. A., de Lencastre, H. (2007). Characterization of Staphylococcus aureus Isolates from Buffalo, Bovine, Ovine, and Caprine Milk Samples Collected in Rio de Janeiro State, Brazil. Appl. Environ. Microbiol. 73: 3845-3849 [Abstract] [Full Text]  
  • Anderson, K. L., Lyman, R. L. (2006). Long-Term Persistence of Specific Genetic Types of Mastitis-Causing Staphylococcus aureus on Three Dairies. J DAIRY SCI 89: 4551-4556 [Abstract] [Full Text]  
  • Jorgensen, H. J., Mork, T., Caugant, D. A., Kearns, A., Rorvik, L. M. (2005). Genetic Variation among Staphylococcus aureus Strains from Norwegian Bulk Milk. Appl. Environ. Microbiol. 71: 8352-8361 [Abstract] [Full Text]  
  • Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E., Dowson, C. G. (2005). Multilocus Sequence Typing of Staphylococcus aureus Isolated from High-Somatic-Cell-Count Cows and the Environment of an Organic Dairy Farm in the United Kingdom. J. Clin. Microbiol. 43: 4731-4736 [Abstract] [Full Text]  
  • Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E., Fox, L. K., Schukken, Y. H., Kruze, J. V., Bradley, A. J., Zadoks, R. N., Dowson, C. G. (2005). Multilocus Sequence Typing of Intercontinental Bovine Staphylococcus aureus Isolates. J. Clin. Microbiol. 43: 4737-4743 [Abstract] [Full Text]  
  • Guler, L., Ok, U., Gunduz, K., Gulcu, Y., Hadimli, H. H. (2005). Antimicrobial Susceptibility and Coagulase Gene Typing of Staphylococcus aureus Isolated from Bovine Clinical Mastitis Cases in Turkey. J DAIRY SCI 88: 3149-3154 [Abstract] [Full Text]  
  • Mork, T., Tollersrud, T., Kvitle, B., Jorgensen, H. J., Waage, S. (2005). Comparison of Staphylococcus aureus Genotypes Recovered from Cases of Bovine, Ovine, and Caprine Mastitis. J. Clin. Microbiol. 43: 3979-3984 [Abstract] [Full Text]  
  • van Leeuwen, W. B., Melles, D. C., Alaidan, A., Al-Ahdal, M., Boelens, H. A. M., Snijders, S. V., Wertheim, H., van Duijkeren, E., Peeters, J. K., van der Spek, P. J., Gorkink, R., Simons, G., Verbrugh, H. A., van Belkum, A. (2005). Host- and Tissue-Specific Pathogenic Traits of Staphylococcus aureus. J. Bacteriol. 187: 4584-4591 [Abstract] [Full Text]  
  • Sukhnanand, S., Dogan, B., Ayodele, M. O., Zadoks, R. N., Craver, M. P. J., Dumas, N. B., Schukken, Y. H., Boor, K. J., Wiedmann, M. (2005). Molecular Subtyping and Characterization of Bovine and Human Streptococcus agalactiae Isolates. J. Clin. Microbiol. 43: 1177-1186 [Abstract] [Full Text]  
  • Sabour, P. M., Gill, J. J., Lepp, D., Pacan, J. C., Ahmed, R., Dingwell, R., Leslie, K. (2004). Molecular Typing and Distribution of Staphylococcus aureus Isolates in Eastern Canadian Dairy Herds. J. Clin. Microbiol. 42: 3449-3455 [Abstract] [Full Text]  
  • Gilot, P., van Leeuwen, W. (2004). Comparative Analysis of agr Locus Diversification and Overall Genetic Variability among Bovine and Human Staphylococcus aureus Isolates. J. Clin. Microbiol. 42: 1265-1269 [Abstract] [Full Text]  
  • Boerlin, P., Kuhnert, P., Hussy, D., Schaellibaum, M. (2003). Methods for Identification of Staphylococcus aureus Isolates in Cases of Bovine Mastitis. J. Clin. Microbiol. 41: 767-771 [Abstract] [Full Text]  
  • Zadoks, R. N., van Leeuwen, W. B., Kreft, D., Fox, L. K., Barkema, H. W., Schukken, Y. H., van Belkum, A. (2002). Comparison of Staphylococcus aureus Isolates from Bovine and Human Skin, Milking Equipment, and Bovine Milk by Phage Typing, Pulsed-Field Gel Electrophoresis, and Binary Typing. J. Clin. Microbiol. 40: 3894-3902 [Abstract] [Full Text]  
  • Gilot, P., Lina, G., Cochard, T., Poutrel, B. (2002). Analysis of the Genetic Variability of Genes Encoding the RNA III-Activating Components Agr and TRAP in a Population of Staphylococcus aureus Strains Isolated from Cows with Mastitis. J. Clin. Microbiol. 40: 4060-4067 [Abstract] [Full Text]  
  • Amonsin, A., Wellehan, J. F. X., Li, L.-L., Laber, J., Kapur, V. (2002). DNA Fingerprinting of Pasteurella multocida Recovered from Avian Sources. J. Clin. Microbiol. 40: 3025-3031 [Abstract] [Full Text]  
  • Herron, L. L., Chakravarty, R., Dwan, C., Fitzgerald, J. R., Musser, J. M., Retzel, E., Kapur, V. (2002). Genome Sequence Survey Identifies Unique Sequences and Key Virulence Genes with Unusual Rates of Amino Acid Substitution in Bovine Staphylococcus aureus. Infect. Immun. 70: 3978-3981 [Abstract] [Full Text]  
  • Fitzgerald, J. R., Sturdevant, D. E., Mackie, S. M., Gill, S. R., Musser, J. M. (2001). Evolutionary genomics of Staphylococcus aureus: Insights into the origin of methicillin-resistant strains and the toxic shock syndrome epidemic. Proc. Natl. Acad. Sci. USA 10.1073/pnas.161098098v1 [Abstract] [Full Text]  
  • Jeffers, G. T., Bruce, J. L., McDonough, P. L., Scarlett, J., Boor, K. J., Wiedmann, M. (2001). Comparative genetic characterization of Listeria monocytogenes isolates from human and animal listeriosis cases. Microbiology 147: 1095-1104 [Abstract] [Full Text]  
  • Zadoks, R., van Leeuwen, W., Barkema, H., Sampimon, O., Verbrugh, H., Schukken, Y. H., van Belkum, A. (2000). Application of Pulsed-Field Gel Electrophoresis and Binary Typing as Tools in Veterinary Clinical Microbiology and Molecular Epidemiologic Analysis of Bovine and Human Staphylococcus aureus Isolates. J. Clin. Microbiol. 38: 1931-1939 [Abstract] [Full Text]  
  • Iannelli, D., D'Apice, L., Fenizia, D., Serpe, L., Cottone, C., Viscardi, M., Capparelli, R. (1998). Simultaneous Identification of Antibodies to Brucella abortus and Staphylococcus aureus in Milk Samples by Flow Cytometry. J. Clin. Microbiol. 36: 802-806 [Abstract] [Full Text]  
  • Fitzgerald, J. R., Sturdevant, D. E., Mackie, S. M., Gill, S. R., Musser, J. M. (2001). Evolutionary genomics of Staphylococcus aureus: Insights into the origin of methicillin-resistant strains and the toxic shock syndrome epidemic. Proc. Natl. Acad. Sci. USA 98: 8821-8826 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»