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Journal of Clinical Microbiology, Feb 1995, 445-454, Vol 33, No. 2
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice

JG Fox, LL Yan, FE Dewhirst, BJ Paster, B Shames, JC Murphy, A Hayward, JC Belcher and EN Mendes
Division of Comparative Medicine, Massachusetts Institute of Technology Cambridge 02139.

A fusiform bacterium with 3 to 14 multiple bipolar sheathed flagella and periplasmic fibers wrapped around the cell was isolated from the liver, bile, and lower intestine of aged, inbred mice. The bacteria grew at 37 and 42 degrees C under microaerophilic conditions, rapidly hydrolyzed urea, were catalase and oxidase positive, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and were resistant to both cephalothin and nalidixic acid but sensitive to metronidazole. On the basis of 16S rRNA gene sequence analysis, the organism was classified as a novel helicobacter, Helicobacter bilis. This new helicobacter, like Helicobacter hepaticus, colonizes the bile, liver, and intestine of mice. Although the organism is associated with multifocal chronic hepatitis, further studies are required to ascertain whether H. bilis is responsible for causing chronic hepatitis and/or hepatocellular tumors in mice.

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• Baker, D. G. (1998). Natural Pathogens of Laboratory Mice, Rats, and Rabbits and Their Effects on Research. Clin. Microbiol. Rev. 11: 231-266 [Abstract] [Full Text]