This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McCullough, M. Articles by Reade, P. Search for Related Content PubMed PubMed Citation Articles by McCullough, M. Articles by Reade, P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 03 1995, 696-700, Vol 33, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Characterization of genetically distinct subgroup of Candida albicans strains isolated from oral cavities of patients infected with human immunodeficiency virus

M McCullough, B Ross and P Reade
School of Dental Science, Faculty of Medicine, Dentistry, and Health Science, University of Melbourne, Victoria, Australia.

During the course of a study of oral Candida albicans strains from 60 human immunodeficiency virus-infected patients over a 2.5-year period, 18 of the 295 C. albicans isolates had genomes that failed to hybridize with a C. albicans-specific DNA probe (27A). These strains were germ tube positive and chlamydospore positive and were identified as C. albicans by the ID 32C test (API Systems, Montlieu, France). These strains were analyzed for the presence of two other C. albicans- specific DNA segments by PCR. The first was a C. albicans 1,348-bp species-specific sequence, and the second was a 1,059-bp C. albicans repetitive element. The probe 27A-hybridizing strains yielded PCR products which differed from those of the nonhybridizing strains. Five of these genetically atypical C. albicans strains and 98 of the C. albicans strains were then analyzed for purported virulence factors. The genetically atypical C. albicans strains, in comparison with typical C. albicans strains, produced greater amounts of extracellular proteinase (P = 0.038, Student's t test), adhered to a greater degree to buccal epithelial cells (P = 0.018, Student's t test), and were less susceptible to the antifungal drug 5-flucytosine (P = 0.0003, Mann- Whitney test). Analysis of these strains with other common antifungal drugs showed no statistically significant variation in susceptibility. The results of this study indicated that these genetically atypical C. albicans strains possess increased virulence in comparison with typical C. albicans strains.

This article has been cited by other articles:

• Coogan, M.M., Fidel, P.L. Jr., Komesu, M.C., Maeda, N., Samaranayake, L.P. (2006). (B1) Candida and Mycotic Infections. Adv. Dent. Res. 19: 130-138 [Abstract] [Full Text]  
• Marot-Leblond, A., Beucher, B., David, S., Nail-Billaud, S., Robert, R. (2006). Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies. J. Clin. Microbiol. 44: 138-142 [Abstract] [Full Text]  
• Marot-Leblond, A., Grimaud, L., David, S., Sullivan, D. J., Coleman, D. C., Ponton, J., Robert, R. (2004). Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis. J. Clin. Microbiol. 42: 4956-4960 [Abstract] [Full Text]  
• Pujol, C., Daniels, K. J., Lockhart, S. R., Srikantha, T., Radke, J. B., Geiger, J., Soll, D. R. (2004). The Closely Related Species Candida albicans and Candida dubliniensis Can Mate. Eukaryot Cell 3: 1015-1027 [Abstract] [Full Text]  
• Hira, S. K. (2003). Occurrence and Management of Opportunistic Infections Associated with HIV/AIDS in Asia. Journal of Health Management 5: 237-260 [Abstract]  
• Fotedar, R., Al Hedaithy, S. S. A. (2003). Candida dubliniensis at a University Hospital in Saudi Arabia. J. Clin. Microbiol. 41: 1907-1911 [Abstract] [Full Text]  
• Gee, S. F., Joly, S., Soll, D. R., Meis, J. F. G. M., Verweij, P. E., Polacheck, I., Sullivan, D. J., Coleman, D. C. (2002). Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo. J. Clin. Microbiol. 40: 556-574 [Abstract] [Full Text]  
• Jabra-Rizk, M. A., Ferreira, S. M. S., Sabet, M., Falkler, W. A., Merz, W. G., Meiller, T. F. (2001). Recovery of Candida dubliniensis and Other Yeasts from Human Immunodeficiency Virus-Associated Periodontal Lesions. J. Clin. Microbiol. 39: 4520-4522 [Abstract] [Full Text]  
• Staib, P., Moran, G. P., Sullivan, D. J., Coleman, D. C., Morschhäuser, J. (2001). Isogenic Strain Construction and Gene Targeting in Candida dubliniensis. J. Bacteriol. 183: 2859-2865 [Abstract] [Full Text]  
• Hazen, K. C., Wu, J. G., Masuoka, J. (2001). Comparison of the Hydrophobic Properties of Candida albicans and Candida dubliniensis. Infect. Immun. 69: 779-786 [Abstract] [Full Text]  
• Jabra-Rizk, M. A., Falkler, W. A. Jr., Merz, W. G., Baqui, A. A. M. A., Kelley, J. I., Meiller, T. F. (2000). Retrospective Identification and Characterization of Candida dubliniensis Isolates among Candida albicans Clinical Laboratory Isolates from Human Immunodeficiency Virus (HIV)-Infected and Non-HIV-Infected Individuals. J. Clin. Microbiol. 38: 2423-2426 [Abstract] [Full Text]  
• Polacheck, I., Strahilevitz, J., Sullivan, D., Donnelly, S., Salkin, I. F., Coleman, D. C. (2000). Recovery of Candida dubliniensis from Non-Human Immunodeficiency Virus-Infected Patients in Israel. J. Clin. Microbiol. 38: 170-174 [Abstract] [Full Text]  
• Pincus, D. H., Coleman, D. C., Pruitt, W. R., Padhye, A. A., Salkin, I. F., Geimer, M., Bassel, A., Sullivan, D. J., Clarke, M., Hearn, V. (1999). Rapid Identification of Candida dubliniensis with Commercial Yeast Identification Systems. J. Clin. Microbiol. 37: 3533-3539 [Abstract] [Full Text]  
• Jabra-Rizk, M. A., Falkler, W. A. Jr., Merz, W. G., Kelley, J. I., Baqui, A. A. M. A., Meiller, T. F. (1999). Coaggregation of Candida dubliniensis with Fusobacterium nucleatum. J. Clin. Microbiol. 37: 1464-1468 [Abstract] [Full Text]  
• Joly, S., Pujol, C., Rysz, M., Vargas, K., Soll, D. R. (1999). Development and Characterization of Complex DNA Fingerprinting Probes for the Infectious Yeast Candida dubliniensis. J. Clin. Microbiol. 37: 1035-1044 [Abstract] [Full Text]  
• Jabra-Rizk, M. A., Baqui, A. A. M. A., Kelley, J. I., Falkler, W. A. Jr., Merz, W. G., Meiller, T. F. (1999). Identification of Candida dubliniensis in a Prospective Study of Patients in the United States. J. Clin. Microbiol. 37: 321-326 [Abstract] [Full Text]  
• McCullough, M. J., Clemons, K. V., Stevens, D. A. (1999). Molecular and Phenotypic Characterization of Genotypic Candida albicans Subgroups and Comparison with Candida dubliniensis and Candida stellatoidea. J. Clin. Microbiol. 37: 417-421 [Abstract] [Full Text]  
• Moran, G. P., Sanglard, D., Donnelly, S. M., Shanley, D. B., Sullivan, D. J., Coleman, D. C. (1998). Identification and Expression of Multidrug Transporters Responsible for Fluconazole Resistance in Candida dubliniensis. Antimicrob. Agents Chemother. 42: 1819-1830 [Abstract] [Full Text]  
• Sullivan, D., Coleman, D. (1998). Candida dubliniensis: Characteristics and Identification. J. Clin. Microbiol. 36: 329-334 [Full Text]  
• Timmins, E. M., Howell, S. A., Alsberg, B. K., Noble, W. C., Goodacre, R. (1998). Rapid Differentiation of Closely Related Candida Species and Strains by Pyrolysis-Mass Spectrometry and Fourier Transform-Infrared Spectroscopy. J. Clin. Microbiol. 36: 367-374 [Abstract] [Full Text]