This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Allen, R. D. Articles by Koopmans, M. Search for Related Content PubMed PubMed Citation Articles by Allen, R. D. Articles by Koopmans, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, Mar 1995, 725-728, Vol 33, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA

RD Allen, PE Pellett, JA Stewart and M Koopmans
Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.

This article has been cited by other articles:

• Habbal, W., Monem, F., Gartner, B. C. (2005). Errors in Published Sequences of Human Cytomegalovirus Primers and Probes: Do We Need More Quality Control?. J. Clin. Microbiol. 43: 5408-5409 [Full Text]  
• Boriskin, Y. S., Rice, P. S., Stabler, R. A., Hinds, J., Al-Ghusein, H., Vass, K., Butcher, P. D. (2004). DNA Microarrays for Virus Detection in Cases of Central Nervous System Infection. J. Clin. Microbiol. 42: 5811-5818 [Abstract] [Full Text]  
• Hong, Y., Berrang, M. E., Liu, T., Hofacre, C. L., Sanchez, S., Wang, L., Maurer, J. J. (2003). Rapid Detection of Campylobacter coli, C. jejuni, and Salmonella enterica on Poultry Carcasses by Using PCR-Enzyme-Linked Immunosorbent Assay. Appl. Environ. Microbiol. 69: 3492-3499 [Abstract] [Full Text]  
• Boom, R., Sol, C., Weel, J., Gerrits, Y., de Boer, M., Wertheim-van Dillen, P. (1999). A Highly Sensitive Assay for Detection and Quantitation of Human Cytomegalovirus DNA in Serum and Plasma by PCR and Electrochemiluminescence. J. Clin. Microbiol. 37: 1489-1497 [Abstract] [Full Text]