JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sorg, I.
Right arrow Articles by Metzler, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sorg, I.
Right arrow Articles by Metzler, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, 04 1995, 821-823, Vol 33, No. 4
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Detection of Borna disease virus RNA in formalin-fixed, paraffin- embedded brain tissues by nested PCR

I Sorg and A Metzler
Institute of Parasitology, Veterinary Medical Faculty, University of Zurich, Switzerland.

A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1995 by the American Society for Microbiology. All rights reserved.